This is the first report to investigate the antifungal susceptibility of 21 clinical isolates of seven Candida species to epigallocatechin 3-O-gallate (EGCg) and to compare with six antifungal agents, amphotericin B (AMPH), fluconazole (FLCZ), flucytosin (5FC), itraconazole (ITCZ), micafungin (MCFG), and miconazole (MCZ), using a method following the National Committee for Clinical Laboratory Standards (NCCLS) M27-A guidelines. Among the tested species, Candida glabrata exhibited the highest susceptibility to EGCg (MIC50, 0.5-1 mu g/ml and MIC90, 1-2 mu g/ml) compared favorably with FLCZ, although they were slightly less susceptible than to AMPH, 5FC, MCFG. ITCZ. and MCZ. Candida guilliemondii and Candida parapsilosis (MIC50, 1-4 mu g/ml and MIC90, 2-16 mu g/ml) were also susceptible to EGCg, although they appear to be slightly less susceptible to EGCg than C. glabrata and the other antifungal agents tested. Moreover. the susceptibility of Candida krusei strains (MIC50, 2 mu g/ml and MIC90, 4-8 mu g/ml) to EGCg was approximately 2- to 8-fold higher than those of 5FC and FLCZ. Our data indicate that EGCg can inhibit clinically pathogenic Candida species, although the concentrations of EGCg for antifungal susceptibility were slightly higher than those of tested antifungal agents on the whole. Based on these results, we suggest that EGCg may be effectively used as a possible agent or adjuvant for antifungal therapy in Candidiasis. (c) 2006 Elsevier Inc. All rights reserved.