Biochemical and Biophysical Research Communications, Vol.283, No.4, 896-899, 2001
Novel inhibitors of HIV-reverse transcriptase catalyzed DNA strand transfer: Can we alter the infidelity of the enzyme?
Reverse transcription of viral RNA by the enzyme HIV1-reverse transcriptase (HIV1-RT) involves two DNA-strand transfers (DNA-ST). We report here a time-dependent inactivation of DNA-strand transfer by specific DNA-strand transfer inhibitors that do not affect the polymerase activity of the enzyme. Irreversible inhibitors of this type may be useful in characterizing the sites on the enzyme responsible for DNA-Strand transfer.