화학공학소재연구정보센터
Biochemical and Biophysical Research Communications, Vol.285, No.3, 702-707, 2001
Kinetics study of a selenium-containing ScFv catalytic antibody that mimics glutathione peroxidase
The steady state kinetic study and some enzymic characterization of a selenium-containing scFv catalytic antibody (Se-scFv2F3) was carried out. A novel reaction formula of this abzyme-catalyzed reaction was proposed and a rate equation was gotten according to the formula. The constants in the equation were compared with Dalziel's parameters and the exact meanings of these constants were analyzed. The gotten kinetics parameters from the kinetics study of Se-scFv2F3 were analyzed and compared with that of native glutathione peroxidase.