γ-벤질 L-글루타메이트/프로필렌 옥사이드 블록 공중합체의 항혈전성에 관한 in vitro와 in vivo에서의 연구

조종수; 송수창; 김주오; 김상수; 양동욱; 김계용; 성용길; 양흥현; 김성완

Polymer(Korea), Vol.14, No.6, 622-629, December, 1990

Export Citation

γ-벤질 L-글루타메이트/프로필렌 옥사이드 블록 공중합체의 항혈전성에 관한 in vitro와 in vivo에서의 연구

In Vitro and in Vivo Studies on Antithrombogenicity of γ-Benzyl L-Glutamate-Propylene Oxide Block Copolymer

조종수, 송수창, 김주오, 김상수, 양동욱, 김계용, 성용길, 양흥현, 김성완

초록

폴리 γ-벤질 L-글루타메이트/폴리 프로필렌 옥사이드 블록 공중합체의 항혈전성에 대하여 in vitro와 in vivo에서 행해졌다. in vitro에서의 혈소판 점착실험은 미립자칼림방법이 채택되었고, in vivo에서의 항혈전성은 블록공중합체로 코팅된 1.66mm의 작은 직경의 catheter을 개의 정맥혈관에 삽입한 후 일정한 시간에서 형성된 혈전량을 가지고 평가하였다. in vitro 실험결과 혈소판의 점착은 미크로상분리구조와 2차 구조를 갖는 블록공중합체에서 억제되었다. in vivo 실험결과에서도 블록공중합체에서 폴리우레탄에 비해 월등한 항혈전성을 나타내었다.

In vitro and in vivo antithrombogenicity of poly(γ-benzy1 L-glutamate)/poly( propylene oxide) block copolymer was studied. For in vitro evaluation of platelet adhesion on block copolymer surfaces, a microsphere column method was used. For in vivo evaluation of the antithrombogenicity of copolymer surfaces, small diameter(I.D. : 1.66mm) catheter precoated with copolymer was implanted in dogs to determine the mean thrombus weight. In vitro test showed that platelet adhesion was suppressed on block copolymers having microphase-separated structure. In vitro test showed that block copolymer surface exhibited excellent antithrombogenicity as compared with polyurethane catheter.

[References]

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Kim SW, Edert CD, Lin JY, McRea JC, ASHIO, 6, 76 (1983)
Heyman PW, Cho CS, McRea JC, Olsen DB, Kim SW, J. Biomed. Mater. Res., 19, 419 (1985)
Lyman DJ, Knutson K, McNeill B, Shipbtani K, Trans. Am. Soc. Artif. Int. Organs., 21, 49 (1975)
Okano T, Nishiyama S, Shinohara I, Akaike T, Sakurai Y, Kataoka K, Tsuruta T, J. Biomed. Mater. Res., 15, 393 (1981)
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Kang I, Ito Y, Sisido M, Imanishi Y, Polym. J., 19, 1329 (1987)
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Cho CS, Kim SW, Sung YK, Kim KY, Makromol. Chem., 189, 1505 (1988)
Fox HY, Zisman WA, J. Colloid Sci., 7, 109 (1952)
Cho CS, private communication
Yui N, Kataoka K, Sakurai Y, Aoki T, Sanui K, Ogata N, Biomaterials, 9, 225 (1988)
Yui N, Sanui K, Ogata N, Kataoka K, Okano T, Sakurai Y, J. Biomed. Mater. Res., 20, 929 (1986)
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Colman RW, Hirsh J, Marder VJ, Salzman JB, Hemostasis and Thrombosis, Lippincott, Pennsylvania (1983)

[1] Andrade JD, Med. Instrum., 7, 110 (1973)

[2] Okano T, Nishiyama S, Shinohara I, Akaike T, Sakurai Y, Kataoka T, Tsuruta T, Kuwana K, Miyata S, Artif. Organs., 3(S), 253 (1979)

[3] Kim SW, Edert CD, Lin JY, McRea JC, ASHIO, 6, 76 (1983)

[4] Heyman PW, Cho CS, McRea JC, Olsen DB, Kim SW, J. Biomed. Mater. Res., 19, 419 (1985)

[5] Lyman DJ, Knutson K, McNeill B, Shipbtani K, Trans. Am. Soc. Artif. Int. Organs., 21, 49 (1975)

[6] Okano T, Nishiyama S, Shinohara I, Akaike T, Sakurai Y, Kataoka K, Tsuruta T, J. Biomed. Mater. Res., 15, 393 (1981)

[7] Barenberg SA, Schultz JS, Anderson JM, Geil PH, Trans. Am. Soc. Artif. Intern. Organs., 25, 159 (1979)

[8] Nakajima A, Kugo K, Hayashi T, Polym. J., 11, 995 (1979)

[9] Kang I, Ito Y, Sisido M, Imanishi Y, Polym. J., 19, 1329 (1987)

[10] Cho CS, Song SC, Kim SU, Ryang DW, Sung YK, Kim KY, J. Korean Soc. Medical Biological Eng., 8, 199 (1987)

[11] Cho CS, Kim SW, Sung YK, Kim KY, Makromol. Chem., 189, 1505 (1988)

[12] Fox HY, Zisman WA, J. Colloid Sci., 7, 109 (1952)

[13] Cho CS, private communication

[14] Yui N, Kataoka K, Sakurai Y, Aoki T, Sanui K, Ogata N, Biomaterials, 9, 225 (1988)

[15] Yui N, Sanui K, Ogata N, Kataoka K, Okano T, Sakurai Y, J. Biomed. Mater. Res., 20, 929 (1986)

[16] Okano T, Uruno M, Sugiyama N, Shimada M, Shinohara I, Kataoka K, Sakurai Y, J. Biomed. Mater. Res., 20, 1035 (1986)

[17] Colman RW, Hirsh J, Marder VJ, Salzman JB, Hemostasis and Thrombosis, Lippincott, Pennsylvania (1983)