Electrophoresis, Vol.21, No.1, 211-219, 2000
High-speed chiral separations on microchip electrophoresis devices
High-speed electrophoretic chiral separations have been successfully performed in a of microfabricated device by employing cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC). Utilizing short separation channels and relatively high field strengths in combination with small volume-defined injection plugs, and operating in counter-electroosmotic flow conditions, fast and efficient separations of fluorescein insothiocyanate (FITC)-labeled amino acid enantiomers were obtained. Analysis time ranged from 75 s for the most basic amino acids to 160 s for the most acidic ones with associated efficiencies from 7000 up to 28 000 effective plates (100 000 to 395 000 plates/m). Buffer parameters were varied in order to study the effect on chiral resolution. A buffer system consisting of 100 mM berate (pH 9.4), 30 mM of SDS, and 10 mM gamma-CD as chiral selector provided adequate resolution of the majority of FITC-amino acid enantiomers tested.
Keywords:microchip electrophoresis;chiral separation of amino acids;fluorescein isothiocyanate;cyclodextrins;micellar electrokinetic chromatography