Active carboxylic acid-terminated alkanethiol self-assembled monolayers on gold bead electrodes for immobilization of cytochromes c

Tanimura R; Hill MG; Margoliash E; Niki K; Ohno H; Gray HB

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Electrochemical and Solid State Letters, Vol.5, No.12, E67-E70, 2002

Active carboxylic acid-terminated alkanethiol self-assembled monolayers on gold bead electrodes for immobilization of cytochromes c

Tanimura R, Hill MG, Margoliash E, Niki K, Ohno H, Gray HB

It is extremely difficult to immobilize cytochrome c (cyt c) on carboxylic acid-terminated alkanethiol self-assembled monolayers (HOOC-SAM) on gold bead electrodes prepared in a hydrogen flame. We found that simple pretreatment of a HOOC-SAM/gold bead electrode by potential cycling in buffer solution in the range +/-300 mV prior to immobilization of the protein facilitated stable cyt c binding to HOOC-SAMs. The stability of cyt c on the HOOC-SAMs is independent of the topology of the gold surface. (C) 2002 The Electrochemical Society.