Bacteria isolated from biofilms of water distribution pipes and colonized catheters from hospitalized patients were studied for their haemagglutination ability, expression of lectins and hydrophobicity. Higher haemagglutination ability of clinical strains for human red blood cells was demonstrated, which could be an expression of their adaptation to the human ecosystem. Environmental strains had higher hydrophobicity, possibly related to adaptation to a low nutritive ecosystem. Expression of lectins was relatively low and comparable in both bacterial populations, but carbohydrate specificities were very different, possibly related to a different implication of these structures in the two ecosystems.