Aims: The aim of this study was to evaluate the potential of Listeria monocytogenes to grow in mushrooms packaged in two different types of PVC films when stored at 4 degreesC and 10 degreesC. Methods and Results: Mushrooms were packed in two polymeric films (perforated and nonperforated PVC) and stored at 4 degreesC and 10 degreesC. The carbon dioxide and oxygen content inside the packages, aerobic mesophiles, psychrotrophs, Pseudomonas spp., Listeria monocytogenes, faecal coliforms, Escherichia coli, anaerobic spores and major sensory factors were determined. The mushrooms packaged in nonperforated film and stored at 4 degreesC had the most desirable quality parameters (texture, development stage and absence of moulds). Listeria monocytogenes was able to grow at 4 degreesC and 10 degreesC in inoculated mushrooms packaged in perforated and nonperforated films between 1 and 2 log units during the first 48 h. After 10 d of storage, the populations of L. monocytogenes were higher in mushrooms packaged in nonperforated film and stored at 10 degreesC. Conclusions: MAP followed by storage at 4 degreesC or 10 degreesC extends the shelf life by maintaining an acceptable appearance, but allows the growth and survival of L. monocytogenes. Significance and Impact of the Study: According to this study additional hurdles must be studied in order to prevent the growth of L. monocytogenes.