화학공학소재연구정보센터
Journal of Applied Microbiology, Vol.95, No.3, 479-483, 2003
Acclimation and repair of DNA damage in recombinant bioluminescent Escherichia coli
Aims: The aim of this study is to understand different adaptive responses in bacteria caused by three different mutagens, namely, an intercalating agent, an alkylating agent and a hydroxylating agent, and the repair systems according to the type of DNA damage, that is, DNA cross-linking and delayed DNA synthesis, alkylation and hydroxylation of DNA. A recombinant bioluminescent Escherichia coli , DPD2794 with the recA promoter fused to luxCDABE originating from Vibrio fischeri , was used in this study. Methods and Results: The recombinant bioluminescent E. coli strain DPD2794, containing a recA promoter fused to luxCDABE from V. fischeri , was used to detect adaptive and repair responses to DNA damage caused by mitomycin C (MMC), and these responses were compared with those when the cells were induced with N -methyl-N -nitro-N -nitrosoguanidine (MNNG) and hydrogen peroxide (H-2 O-2 ). The response ratio between the induced samples and that of the controls decreased suddenly when the induced culture was used in further inductions, indicating a possible adaptive response to DNA damage. DNA damage, or the proteins produced, because of MMC addition does not appear to be completely resolved until the seventh sub-culture after the initial induction, whereas simple damage, such as the base modification caused by MNNG and H-2 O-2 , appears to be repaired rapidly as evidenced by the quick recovery of sensitivity. Conclusions: These results suggest that it takes more time to completely repair DNA damage caused by MMC, as compared with a simple repair such as that required for the damage caused by MNNG and H-2 O-2 . Therefore, repair of the damage caused by these three mutagens is controlled by different regulons, even though they all induced the recA promoter. Significance and Impact of the Study: Using a bioluminescent E. coli harbouring a recA promoter-lux fusion, it was found that different adaptive responses and repair systems for DNA damage caused by several mutagens exists in E. coli .