The fundamental parameters regarding linear molecular motors have been obtained via single molecule studies for dynamic (e.g. force) and static parameters (e.g. filament flexibility). However, single molecule studies are not trivial, whereas motility assays are less demanding. A computer package-presented in this contribution with its constitutive equations-for the simulation of motility assays that allows the user to input several fundamental parameters (force exerted by the motors, flexibility of filaments, etc.); collect the predicted statistics and compare the simulated statistics with actual ones, can be then a valuable tool for a less demanding hypothesis testing of molecular motors functions. The program (source in C and executable) and future updates can be downloaded from www.bionanoeng.com. (C) 2003 Elsevier B.V. All rights reserved.