In the present study, reverse microemulsion was prepared to regulate the mineralization of calcium phosphate. Calcium chloride and sodium hydrogen phosphate aqueous solution were, respectively, dropped into the mixture of Span 80, Tween 80, and n-heptane to get two kinds of emulsions. n-Buranol was used to adjust the emulsions to transparent state. Calcium phosphate was prepared by adding phosphate microemulsion to Calcium microemulsion. Heating method was used to induce the phase separation. After being washed with acetone and ethanol, the mineralized deposition were studied with scanning electron microscopy (SEM), X-ray diffraction (XRD) and transmission electron microscopy (TEM). The results indicated that the minerals in the deposition were mainly monetite (DCPA) and the nanosized needle-like DCPA exhibited the preferential orientation along 200 direction. (c) 2005 Elsevier B.V. All rights reserved.