Protein chips are not as well established as DNA chips. Compared with DNA chip fabrication, protein chip construction is more difficult. Nickel chloride coated protein chip plates have been developed using a spin coating system. Water and alcohol have been used as solvent and multilayer effects on each specimen have been investigated. In order to fabricate a proper platform for the protein chip, we examined the efficiency and level of protein absorption to the slide in various coating condition. It was found that the multilayer effects with high concentration of NiCl2 film relatively increase the adhesive capability since the density of nickel ion per unit area of the glass slide increases as multilayer is used. The protein adsorption is decreased by the low concentration, holes and cracks created by a single layer coating observed in the SEM images. (c) 2005 Elsevier B.V. All rights reserved.