Thermodynamic Model for Partition Coefficients in the Two Protein Systems

Chang Min Jung; Young Chan Bae; Jae-Jun Kim

Macromolecular Research, Vol.15, No.7, 682-687, December, 2007

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Thermodynamic Model for Partition Coefficients in the Two Protein Systems

Chang Min Jung, Young Chan Bae^†, and Jae-Jun Kim¹

Division of Chemical Engineering and Molecular Thermodynamics Laboratory, Hanyang University, Seoul 133-791, Korea
¹College of Architecture, Hanyang University, Seoul 133-791, Korea

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The equation of state developed herein is predicated on a hard-sphere reference with perturbations introduced via a potential function to account for electrostatic forces and for attraction between protein particles. During this process, the generalized Lennard-Jones (GLJ) pair potential function is employed. The GLJ pair potential function is employed to represent the protein-protein interaction in two-protein systems. Via the use of the relation between the equation of state and the chemical potential, the phase behavior in the aqueous two-protein system can be estimated. The partition coefficients can be obtained via these processes. The calculated values of the coefficients agree fairly well with the experimental data in the given pH and ionic strength range, with no additional adjustable model parameters.

Keywords:phase equilibria;GLJ potential;partition coefficient;precipitation

[References]

Cohn EJ, Edsall JT, Proteins, Amino Acids, and Peptides, Reinhold, New York, 1943 (1943)
Rothstein F, Differential Precipitation of Proteins, Protein Purification Process Engineering, R. G. Harrison, Ed., Dekker, New York 1994, p. 115
Rosenberger F, J. Cryst. Growth, 166, 40 (1996)
Piazza R, Curr. Opin. Colloid Interface Sci., 5, 38 (2000)
Tardieu A, Bonnete F, Finet DS, Vivares D, Acta Crystallogr. Sect. D: Biol. Crystallogr., 58, 1549 (2002)
Broide ML, Tominc TM, Saxowsky MD, Phys. Rev. E: Stat., Nonlinear, Soft Matter Phys., 53, 6325 (1996)
Grimson MJ, J. Chem. Soc.-Faraday Trans., 2, 817 (1983)
Coen CJ, Ph.D. Thesis, Univ. of California, Berkeley (1995)
Coen CJ, Prausnitz JM, Blanch HW, Biotechnol. Bioeng., 53(6), 567 (1997)
Kim IH, Bae YC, Fluid Phase Equilib., 168(2), 201 (2000)
Boublik T, J. Chem. Phys., 53, 417 (1970)
Carnahan NF, Starling KE, J. Chem. Phys., 51, 635 (1969)
Seong JY, Bae YC, Sun YK, J. Power Sources, 157(2), 733 (2006)
Prausnitz JM, Lichtenthaler RN, Azevedo EGD, Molecular Thermodynamics of Fluid Phase Equilibria, Prentice-Hall, Englewood Cliffs, NJ (1986)
Stein PE, Leslie AGW, Finch JT, Turnell WG, McLaughlin PJ, Carrell RW, Nature, 347, 99 (1990)
Blake CCF, Koenig DF, Mair GA, North ACT, Phillips DC, Sarma VR, Nature, 206, 757 (1965)

[Referenced By]

[Related Articles]

[1] Cohn EJ, Edsall JT, Proteins, Amino Acids, and Peptides, Reinhold, New York, 1943 (1943)

[2] Rothstein F, Differential Precipitation of Proteins, Protein Purification Process Engineering, R. G. Harrison, Ed., Dekker, New York 1994, p. 115

[3] Rosenberger F, J. Cryst. Growth, 166, 40 (1996)

[4] Piazza R, Curr. Opin. Colloid Interface Sci., 5, 38 (2000)

[5] Tardieu A, Bonnete F, Finet DS, Vivares D, Acta Crystallogr. Sect. D: Biol. Crystallogr., 58, 1549 (2002)

[6] Broide ML, Tominc TM, Saxowsky MD, Phys. Rev. E: Stat., Nonlinear, Soft Matter Phys., 53, 6325 (1996)

[7] Grimson MJ, J. Chem. Soc.-Faraday Trans., 2, 817 (1983)

[8] Coen CJ, Ph.D. Thesis, Univ. of California, Berkeley (1995)

[9] Coen CJ, Prausnitz JM, Blanch HW, Biotechnol. Bioeng., 53(6), 567 (1997)

[10] Kim IH, Bae YC, Fluid Phase Equilib., 168(2), 201 (2000)

[11] Boublik T, J. Chem. Phys., 53, 417 (1970)

[12] Carnahan NF, Starling KE, J. Chem. Phys., 51, 635 (1969)

[13] Seong JY, Bae YC, Sun YK, J. Power Sources, 157(2), 733 (2006)

[14] Prausnitz JM, Lichtenthaler RN, Azevedo EGD, Molecular Thermodynamics of Fluid Phase Equilibria, Prentice-Hall, Englewood Cliffs, NJ (1986)

[15] Stein PE, Leslie AGW, Finch JT, Turnell WG, McLaughlin PJ, Carrell RW, Nature, 347, 99 (1990)

[16] Blake CCF, Koenig DF, Mair GA, North ACT, Phillips DC, Sarma VR, Nature, 206, 757 (1965)