Lactobacillus delbrueckii was grown on sugarcane molasses, sugarcane juice and sugar beet juice in batch fermentation at pH 6 and at 40 degrees C. After 72 h, the lactic acid from 13% (w/v) sugarcane molasses (119 g total sugar l(-1)) and sugarcane juice (133 g total sugar l(-1)) was 107 g l(-1) and 120 g l(-1) respectively. With 10%(w/v) sugar beet juice (105 g total sugar l(-1)) 84 g lactic acid l(-1) stop was produced. The optical purities of D-lactic acid from the feedstocks ranged from 97.2 to 98.3%.