A stable analogue of Ala-tRNAAla containing 1,2,4 oxadiazole as a mime of the 3' amino acyl ester was synthesized and showed to inhibit FemX(wv), IC50 = 1.4 M-mu), an alanyl transferase essential to form the peptidoglycan network of Gram-positive bacteria] pathogens. The oxadiazole ring is a good candidate for design of nucleotide analogues which may be applicable to important targets including amino acyl-tRNA synthetases and the ribosomal peptidyl-transferase center.