In this paper the immobilization of glucoamylase and trypsin on thermosensitive copolymers of N-isopropylacrylamide and 2-hydroxyethyl methacrylate or glycidyl methacrylate modified by ainination is evaluated. The method is based on the swelling properties of these stimuli-sensitive polymers. On cooling the polymers expand and work like a pump that sucks up the enzyme adsorbed onto their surface and then the enzyme is crosslinked with glutaraldehyde, thus forming an interpenetrated polymer network. The attention was focused on the properties of the carrier-enzyme systems, particularly on the effect of cross-linking on their storage stability. The data obtained for unmodified thermosensitive carriers and carriers hydrophilised with ethylenediamine were compared. In the case of both enzymes, TH8-NH2 carrier is the most suitable, as the highest activity of immobilized enzymes is retained. Low molecular weight substrates are preferred in both cases. (c) 2007 Elsevier Inc. All rights reserved.