This manuscript demonstrates the possibility of creating affinity sites in polyaniline (PAN) through molecular imprinting for clinically relevant molecules namely, Adenosine triphosphate (ATP), Adenosine diphosphate (ADP) and Adenosine mono phosphate (AMP). The binding of these molecules by the substrate was demonstrated indicating the feasibility of enriching multiple components at a time enabling the subsequent estimation. ATP, ADP and AMP were used as template molecule and aniline as the monomer. The equilibrium uptake was found to vary as ATP > ADP > AMP.