This communication presents a dynamic model for determination of plant and the enclosed room air temperature in different zones of the micro-climate of a greenhouse. The numerical analysis and the experimental validation of the model have been done for a typical winter day of the month of December in Delhi, India. The parametric study has been presented for the effects of infiltration (number of air changes/hr), heat capacity of plants (isothermal mass) and greenhouse relative humidity (gamma) on plant and enclosed room air temperatures. It is observed that there is a more significant effect of heat capacity of the plant and the relative humidity on the plant temperature than that on the room air temperature. In addition, there is a marginal temperature difference in different zones of the greenhouse. There is fair agreement between the theoretical and experimental observations of room air temperature.