A rigorous model is presented for dynamic simulation of an industrial tray column which uses mass and energy balances together with a proper hydraulic equation. Three different liquid flow regimes can be considered in the industrial tray columns which each of them possesses a specific hydraulic equation. The implemented model by recognizing the type of liquid flow regime is able to use the proper hydraulic equation in the computations. Moreover, by calculating the tray efficiency, the effect of tray geometry on the separation performance is investigated. As the result of this study, the open loop response of the industrial tray column is presented after inputting a step change in reflux flow rate.