Two different high-cell-density cultivation processes based on the mutant Saccharomyces cerevisiae GE-2 for simultaneous production of glutathione and ergosterol were investigated. Compared with keeping the ethanol volumetric concentration at a constant low level, feedback control of glucose feeding rate (F) by keeping the descending rate of ethanol volumetric concentration (Delta E/Delta t) between -0.1% and 0.15% per hour was much more efficient to achieve a high glutathione and ergosterol productivity. This bioprocess overcomes some disadvantages of traditional S. cerevisiae-based cultivation process, especially shortening cultivation period and making the cultivation process steady-going. A classical on or off controller was used to manipulate F to maintain Delta E/Delta t at its set point. The dry cell weight, glutathione yield and ergosterol yield reached 110.0 +/- 2.6 g/l, 2,280 +/- 76 mg/l, and 1,510 +/- 28 mg/l in 32 h, respectively.