The global interest in environmentally friendly material over the past few years has led to the development of new research areas in the field of renewable materials and biocomposites. Within this scope, several researches have been conducted to modify natural fibres aiming at an improved compatibility with polymeric matrices. In this study, fibres from the spadix stem of the date palm tree were treated with sodium hydroxide over different times. The effect of the alkali treatment on the structure, thermal and mechanical behaviour of the fibres was verified through chemical analysis, FTIR spectroscopy, TGA, and tensile testing. Comparing the different alkalisation parameters, promising results are obtained with a 2% sodium hydroxide solution over a treatment time of 2 h.