Ethanol is one of the promising future fuels of Direct Alcohol Fuel Cells (DAFC). The electro-oxidation of ethanol fuel on anode made of carbon-supported Pt-Ru electrode catalysts was carried out in a lab scale direct ethanol fuel cell (DEFC). Cathode used was Pt-black high surface area. The membrane electrode assembly (MEA) was prepared by sandwiching the solid polymer electrolyte membrane, prepared from Nafion (R) (SE-5112, DuPont USA) dispersion, between the anode and cathode. The DEFC was fabricated using the MEA and tested at different catalyst loadings at the electrodes, temperatures and ethanol concentrations. The maximum power density of DEFC for optimized value of ethanol concentration, catalyst loading and temperature were determined. The maximum open circuit voltage (OCV) of 0.815 V, short circuit current density (SCCD) of 27.90 mA/cm(2) and power density of 10.30 mW/cm(2) were obtained for anode (Pt-Ru/C) and cathode (Pt-black) loading of I mg/cm(2) at a temperature of 90 degrees C anode and 60 degrees C cathode for 2M ethanol.