저주파수 초음파를 이용한 Lidocaine Gel의 피부투과 촉진 효과

정대인; 이정우; 안효초; 양재헌; 김태열; 조성완; 김영일; Dae-In Jung; Jung-Woo Lee; Hyo-Cho Ahn; Jae-Heon Yang; Tae-Youl Kim; Seong-Wan Cho; Young-Il Kim

Korean Chemical Engineering Research, Vol.46, No.2, 217-221, April, 2008

Export Citation

저주파수 초음파를 이용한 Lidocaine Gel의 피부투과 촉진 효과

Enhanced Transdermal Permeation Effects of Lidocaine Gel by Low Frequency Ultrasound

정대인, 이정우¹, 안효초², 양재헌², 김태열³, 조성완⁴, 김영일^{4, †}

광주보건대학 물리치료과, 506-701 광주시 광산구 신창동 683-3
¹광주여자대학교 물리치료학과, 506-713 광주시 광산구 산정동 165
²우석대학교 약학대학, 565-701 전북 완주군 삼례읍 후정리 490
³동신대학교 물리치료학과, 520-714 전남 나주시 대호동 252
⁴건양대학교 제약공학과, 320-711 충남 논산시 내동 26

Dae-In Jung, Jung-Woo Lee¹, Hyo-Cho Ahn², Jae-Heon Yang², Tae-Youl Kim³, Seong-Wan Cho⁴, and Young-Il Kim^{4, †}

Department of Physical Therapy, Gwangju Health College, 683-3 Sinchang-dong, Gwangsan-gu, Gwangju 506-701, Korea
¹Department of Physical Therapy, Kwangju Women's University, 165 Sanjung-dong, Gwangsan-gu, Gwangju 506-713, Korea
²College of Parmacy, Woosuk University, 490 Hujeong-ri, Samrye-eup, Wanju-gun, Jeonbuk 565-701, Korea
³Department of Physical Therapy, Dongshin University, 252 Daeho-dong, Naju, Jeonnam 520-714, Korea
⁴Department of Pharmaceutical Engineering, Konyang University, 26 Nae-dong, Nonsan, Chungnam 320-711, Korea

E-mail:

초록

다양한 초음파의 주파수와 강도 등의 물리적 인자에 대한 경피투과 촉진효과를 알아보기 위하여 lidocaine gel을 제조하고 hairless mouse의 피부를 이용하여 경피투과 촉진효과를 평가하였다. Lidocaine을 함유한 겔을 제조하여 안정성을 확인할 수 있었으며, 주파수에 따른 lidocaine의 경피투과량은 500 kHz 초음파의 경피투과량이 크게 증가하였으며, 특히 지속초음파에서 현저히 증가하였다. 초음파의 강도에 따른 lidocaine의 경피투과량은 강도가 높을수록 높게 나타났다. 500 kHz 초음파의 조사는 1 kHz에 비하여 flux를 유의성 있게 증가시켰으며, lag time을 상대적으로 지연되는 양상을 나타내었다. 따라서 현재 임상에서 가장 많이 사용하고 있는 1 kHz 초음파를 이용한 음파영동보다 500 kHz 초음파를 이용한 음파영동이 lidocaine의 투과량 증대를 위한 임상적 응용이 가능할 것으로 사료된다.

To investigate the enhancing effects in transdermal permeation of drug using newly designed ultrasound apparatus of 500 kHz, the transdermal permeation studies through the hairless mouse skin were conducted with lidocaine. The ultrasound apparatus of 500 kHz frequency and transducer were newly developed. The drug permeation studies were performed according to the ultrasound frequencies such as 1 MHz and 500 kHz at 1 W/cm2 in intensity in continuous mode or pulsed mode, respectively. The results on transdermal permeation of lidocaine according to ultrasound intensity showed that the drug permeation increased as the intensity was higher.

Keywords:Lidocaine;Low Frequency Phonophoresis;Transdermal Permeation

[References]

Tachibana K, Tachibana S, J. Pharm., 43(4), 270 (1991)
Zderic V, Clark JI, Martin RW, Cornea Nov., 23(8), 804 (2004)
Yang JH, Kim DK, Kim TY, Int. J. Pharm., 302(1-2), 39 (2005)
Ciccone CD, Leggin BG, Callamaro JJ, Physical therapy, 71(9), 666 (1991)
Monti D, Gianelli R, Chetoni P, Int. J. Pharm., 229(1-2), 131 (2001)
Machet L, Boucaud A, Int. J. Pharm., 243(1-2), 1 (2002)
Bommannan D, Menon GK, Okuyuma H, Pharm. Res., 9(8), 1043 (1992)
Zderic V, Vaezy S, Martin RW, Ultrasound Med. Biol., 28(6), 823 (2002)
Patel GM, Chuang AZ, Kiang E, J. Cataract Refract Surg., 26(5), 690 (2000)
Merino G, Kalia YN, Guy RH, J. Pharm. Sci., 92(6), 1125 (2003)
Mitragotri S, Kost J, Adv. Drug Deliv. Rev., 56(5), 589 (2004)
Williams AR, Ultrasonics, 28(3), 137 (1990)
Moussatov AG, Baker AC, Duck FA, Ultrasonics, 36(8), 893 (1998)
Gvarishvili EP, Dushin NV, Vestn. Oftalmol., 115(4), 19 (1999)
Panova IG, Fateeva VI, Petrishcheva TS, Izv. Akad. Nauk. Ser. Biol., Jul-Aug(4), 487 (1995)
Galinkin JL, Rose JB, Harris K, Anesth. Analg., 94(6), 1484 (2002)
Wallace MS, Ridgeway B, Jun E, Reg. Anesth. Pain. Med., 26(3), 229 (2001)
Becker BM, Helfrich S, Acad. Emerg. Med., 12(4), 276 (2005)
Asano J, Suisha F, Takada M, Biol. Pharm. Bull., 20(3), 288 (1997)
McElnay JC, Matthews MP, Harland R, Br. J. Clin. Pharmacol,, 20(4), 421 (1985)
Burry KA, Patton PE, Hermsmayer K, Am. J. Obstet. Gyneco., 180, 1504 (1999)
Katz NP, Shapiro DE, Herrmann TE, Anesth. Analg., 98(2), 371 (2004)
Kim MJ, Kim YI, Yang JH, Yakhak Hoeji, 47(3), 154 (2003)
Lenart I, Auslander D, Ultrasonics, 18(5), 516 (1980)
Tezel A, Sens A, Mitragotri S, J. Pharm. Sci., 91, 444 (2002)
Meidan VM, Docker MF, Walmsley AD, Pharm. Res., 15(1), 85 (1998)
Tang H, Blankschtein D, Langer R, Pharm. Res., 19(8), 1160 (2002)
Fang JY, Fang CL, Sung KC, J. Pharm., 191(1), 3342 (1999)
Kozanoglu E, Basaran S, Guzel R, Swiss Med. Wkly., 133(23-24), 333 (2003)

[1] Tachibana K, Tachibana S, J. Pharm., 43(4), 270 (1991)

[2] Zderic V, Clark JI, Martin RW, Cornea Nov., 23(8), 804 (2004)

[3] Yang JH, Kim DK, Kim TY, Int. J. Pharm., 302(1-2), 39 (2005)

[4] Ciccone CD, Leggin BG, Callamaro JJ, Physical therapy, 71(9), 666 (1991)

[5] Monti D, Gianelli R, Chetoni P, Int. J. Pharm., 229(1-2), 131 (2001)

[6] Machet L, Boucaud A, Int. J. Pharm., 243(1-2), 1 (2002)

[7] Bommannan D, Menon GK, Okuyuma H, Pharm. Res., 9(8), 1043 (1992)

[8] Zderic V, Vaezy S, Martin RW, Ultrasound Med. Biol., 28(6), 823 (2002)

[9] Patel GM, Chuang AZ, Kiang E, J. Cataract Refract Surg., 26(5), 690 (2000)

[10] Merino G, Kalia YN, Guy RH, J. Pharm. Sci., 92(6), 1125 (2003)

[11] Mitragotri S, Kost J, Adv. Drug Deliv. Rev., 56(5), 589 (2004)

[12] Williams AR, Ultrasonics, 28(3), 137 (1990)

[13] Moussatov AG, Baker AC, Duck FA, Ultrasonics, 36(8), 893 (1998)

[14] Gvarishvili EP, Dushin NV, Vestn. Oftalmol., 115(4), 19 (1999)

[15] Panova IG, Fateeva VI, Petrishcheva TS, Izv. Akad. Nauk. Ser. Biol., Jul-Aug(4), 487 (1995)

[16] Galinkin JL, Rose JB, Harris K, Anesth. Analg., 94(6), 1484 (2002)

[17] Wallace MS, Ridgeway B, Jun E, Reg. Anesth. Pain. Med., 26(3), 229 (2001)

[18] Becker BM, Helfrich S, Acad. Emerg. Med., 12(4), 276 (2005)

[19] Asano J, Suisha F, Takada M, Biol. Pharm. Bull., 20(3), 288 (1997)

[20] McElnay JC, Matthews MP, Harland R, Br. J. Clin. Pharmacol,, 20(4), 421 (1985)

[21] Burry KA, Patton PE, Hermsmayer K, Am. J. Obstet. Gyneco., 180, 1504 (1999)

[22] Katz NP, Shapiro DE, Herrmann TE, Anesth. Analg., 98(2), 371 (2004)

[23] Kim MJ, Kim YI, Yang JH, Yakhak Hoeji, 47(3), 154 (2003)

[24] Lenart I, Auslander D, Ultrasonics, 18(5), 516 (1980)

[25] Tezel A, Sens A, Mitragotri S, J. Pharm. Sci., 91, 444 (2002)

[26] Meidan VM, Docker MF, Walmsley AD, Pharm. Res., 15(1), 85 (1998)

[27] Tang H, Blankschtein D, Langer R, Pharm. Res., 19(8), 1160 (2002)

[28] Fang JY, Fang CL, Sung KC, J. Pharm., 191(1), 3342 (1999)

[29] Kozanoglu E, Basaran S, Guzel R, Swiss Med. Wkly., 133(23-24), 333 (2003)