Analysis and comparison of cloning methods for the preparation of repetitive polypeptides

Hun Su Chu; Cha Yong Choi; Jong In Won

Korean Journal of Chemical Engineering, Vol.25, No.5, 1125-1130, September, 2008

DOI10.1007/s11814-008-0184-1 Export Citation

Analysis and comparison of cloning methods for the preparation of repetitive polypeptides

Hun Su Chu, Cha Yong Choi , and Jong In Won^{1, †}

School of Chemical and Biological Engineering, Seoul National University, Gwanak-gu, Seoul 151-742, Korea
¹Department of Chemical Engineering, Hongik University, Mapo-gu, Seoul 121-791, Korea

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Repetitive polypeptides, defined as a protein consisting primarily of tandemly repeated blocks of amino acid sequence, are widely used biomaterials. These repetitive polypeptides can be used in diverse biological fields including tissue engineering scaffolds, drug delivery systems, biomaterials, and DNA separation systems. The physical/chemical properties of the repetitive polypeptides can be improved by changing the composition of the repeated amino acid sequence. In this study, we introduced genetic methods for the production of repetitive polypeptides. By using recursive directional ligation (RDL) and controlled cloning method (CCM), multimerized genes were cloned and identified. Also, we compared the characteristics of recursive directional ligation (RDL) with those of controlled cloning method (CCM).

Keywords:Repetitive Polypeptide;ELP;Recursive Directional Ligation (RDL);Controlled Cloning Method (CCM)

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Vreeland WN, Destruisseaux C, Karger AE, Analytical Chemistry, 73, 1795 (2001)
Sun Z, Cai J, Korean J. Chem. Eng., 23(4), 607 (2006)
Chilkoti A, Matthew RD, Meyer DE, Advanced Drug Delivery Reviews, 54, 1093 (2002)

[Related Articles]

[1] Dougherty MJ, Kothakota S, Mason TL, Tirrell DA, Fournier MJ, Macromolecules, 26, 1779 (1993)

[2] Trendss CJ, Biotechnol., 8, 309 (1990)

[3] McGrath KP, Tirrell DA, Kawai M, Mason TL, Fournier MJ, Biotechnol. Prog., 6, 188 (1990)

[4] Barron AE, Zuckermann RN, Curr. Opin. Chem. Biol., 3, 681 (1999)

[5] Nagarsekar A, Ghandehari HJ, J. Drug Target., 7, 11 (1999)

[6] Park MS, Choi CY, Won JL, Korean J. Biotechnol, 22, 179 (2007)

[7] Prince JT, McGrath KP, Digirolamo CM, Kaplan DL, Biochemistry, 94, 10879 (1995)

[8] McMillan RA, Lee TAT, Conticello VP, Macromolecules, 32(11), 3643 (1999)

[9] Meyer DE, Chilkoti A, Biomacromolecules, 3(2), 357 (2002)

[10] Won JI, Barron AE, Macromolecules, 35(22), 8281 (2002)

[11] Kim JY, O’Malley S, Mulchandani A, Chen W, Anal. Chem., 77, 2318 (2005)

[12] McPherson DT, Xu J, Urry DW, Protein Expr. Purif., 7, 51 (1996)

[13] Noolandi J, Electrophoresis, 13, 394 (1992)

[14] Noolandi J, Electrophoresis, 14, 680 (1993)

[15] Mayer P, Slater GW, Drouin D, Analytical Chemistry, 66, 1777 (1994)

[16] Vreeland WN, Destruisseaux C, Karger AE, Analytical Chemistry, 73, 1795 (2001)

[17] Sun Z, Cai J, Korean J. Chem. Eng., 23(4), 607 (2006)

[18] Chilkoti A, Matthew RD, Meyer DE, Advanced Drug Delivery Reviews, 54, 1093 (2002)