Magnetic and Temperature-Sensitive Composite Polymer Particles and Adsorption Behavior of Emulsifiers and Trypsin

Hasan Ahmad; M. Abdur Rahman; M. A. Jalil Miah; Klaus Tauer

Macromolecular Research, Vol.16, No.7, 637-643, October, 2008

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Magnetic and Temperature-Sensitive Composite Polymer Particles and Adsorption Behavior of Emulsifiers and Trypsin

Hasan Ahmad^†, M. Abdur Rahman, M. A. Jalil Miah, and Klaus Tauer^{1, †}

Department of Chemistry, Rajshahi University, Rajshahi 6205, Bangladesh
¹MPI of Colloid and Interface, Am M"uhlenberg, D 14476 Golm, Germany

E-mail:,

A combination of magnetic and temperature-responsive properties in the same polymer composites is expected to increase their potential applications in the biomedical field. Accordingly, micron-sized magnetite/polystyrene/poly(2-dimethylaminoethyl methacrylate-ethyleneglycol dimethacrylate), which are abbreviated as Fe3O4/PS/P (DM-EGDM) composite polymer particles, were prepared by the seeded copolymerization of DM and EGDM in the presence of magnetite/polystyrene (Fe3O4/PS) particles. Fe3O4/PS/P(DM-EGDM) composite particles with magnetic properties showed a temperature-sensitive phase transition at approximately 31 oC. The adsorption behavior of the low molecular weight emulsifiers and trypsin (TR) as biomolecules were examined on Fe3O4/PS/P(DM-EGDM) composite polymer particles at different temperatures. The native conformation of TR was followed by measuring the specific activity under various adsorption conditions. The activity of the adsorbed TR on composite polymer particles was higher than those of the free TR and TR adsorbed on Fe3O4/PS particles.

Keywords:Fe3O4;temperature-sensitive;LCST;TR;specific activity

[References]

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Li XH, Sun ZG, J. Appl. Polym. Sci., 58(11), 1991 (1995)
Horak D, Bohacek J, Subrt M, J. Polym. Sci. A: Polym. Chem., 38(7), 1161 (2000)
Cocker TM, Fee CJ, Evans RA, Biotechnol. Bioeng., 53(1), 79 (1997)
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Yang C, Liu H, Guan Y, Xing J, Liu J, Shan G, J.Magn. Magn. Mater., 293, 187 (2005)
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Nakamae TK, Okubo M, Sue M, Shimao M, Komura M, Kobunshi Ronbunshu, 31, 669 (1974)
Okubo M, Ahmad H, J. Polym. Sci. A: Polym. Chem., 34(15), 3147 (1996)
Okubo M, Ahmad H, Colloid Polym. Sci., 274, 112 (1996)
Okubo M, Ahmad H, Colloid Surface A, 153, 429 (1999)
Ahmad H, Okubo M, Kamatari YO, Minami H, Colloid Polym. Sci., 280, 310 (2002)
Alam MA, Miah MAJ, Ahmad H, J. Polym. Mater., 20, 243 (2003)
Alam MA, Miah MAJ, Ahmad H, J. Polym. Mater, J. Polym. Mater., 24, 85 (2007)
Hoffman AS, J. Control. Release, 6, 297 (1987)
Jeong B, Bae YH, Lee DS, Kim SW, Nature, 388(6645), 860 (1997)
Deng YL, Pelton R, Macromolecules, 28(13), 4617 (1995)
Kawaguchi H, Fujimoto K, Bioseparation, 6, 897 (1997)
Miyata T, Asami N, Uragami T, Macromolecules, 32(6), 2082 (1999)
Schmidt AM, Colloid Polym. Sci., 285, 953 (2007)
Sauzedde F, Ela ssari A, Pichot C, Colloid Polym. Sci., 277, 1041 (1999)
Cai J, Guo J, Ji M, Yang W, Wang C, Fu S, Colloid Polym. Sci., 285, 1607 (2007)
Zhu Y, Wu Q, J. Nanopart. Res., 1, 393 (1999)
Tang BZ, Geng Y, Lam JWY, Li B, Jing X, Wang X, Pakhomov AB, Zhang XX, Chem. Mater., 11, 1581 (1999)
Ahmad H, Rahman MS, Miah MAJ, Ali AMI, Colloid Polym. Sci., 279, 1039 (2001)
Zhang JL, Srivastava RS, Misra RDK, Langmuir, 23(11), 6342 (2007)
Ma M, Zhang Y, Yu W, Shen HY, Zhang HQ, Gu N, Colloid Surface A, 212, 219 (2003)
Sayar F, Guven G, Pi kin E, Colloid Polym. Sci., 284, 965 (2006)
Okubo M, Ahmad H, Colloid Polym. Sci., 273, 817 (1995)

[Referenced By]

[1] Noriko Y, Hiromichi N, Hideki A, Tatsuo S, J. Appl.Polym. Sci., 50, 765 (1993)

[2] Li XH, Sun ZG, J. Appl. Polym. Sci., 58(11), 1991 (1995)

[3] Horak D, Bohacek J, Subrt M, J. Polym. Sci. A: Polym. Chem., 38(7), 1161 (2000)

[4] Cocker TM, Fee CJ, Evans RA, Biotechnol. Bioeng., 53(1), 79 (1997)

[5] Lee Y, Rho J, Jung B, J. Appl. Polym. Sci., 89(8), 2058 (2003)

[6] Yang C, Liu H, Guan Y, Xing J, Liu J, Shan G, J.Magn. Magn. Mater., 293, 187 (2005)

[7] Lee J, Senna M, Colloid Polym. Sci., 273, 76 (1995)

[8] Ugelstad J, Ellingsen T, Berge A, et al., PCT Int. Appl. WO Patent 8,303,920 (1983).

[9] Nakamae TK, Okubo M, Sue M, Shimao M, Komura M, Kobunshi Ronbunshu, 31, 669 (1974)

[10] Okubo M, Ahmad H, J. Polym. Sci. A: Polym. Chem., 34(15), 3147 (1996)

[11] Okubo M, Ahmad H, Colloid Polym. Sci., 274, 112 (1996)

[12] Okubo M, Ahmad H, Colloid Surface A, 153, 429 (1999)

[13] Ahmad H, Okubo M, Kamatari YO, Minami H, Colloid Polym. Sci., 280, 310 (2002)

[14] Alam MA, Miah MAJ, Ahmad H, J. Polym. Mater., 20, 243 (2003)

[15] Alam MA, Miah MAJ, Ahmad H, J. Polym. Mater, J. Polym. Mater., 24, 85 (2007)

[16] Hoffman AS, J. Control. Release, 6, 297 (1987)

[17] Jeong B, Bae YH, Lee DS, Kim SW, Nature, 388(6645), 860 (1997)

[18] Deng YL, Pelton R, Macromolecules, 28(13), 4617 (1995)

[19] Kawaguchi H, Fujimoto K, Bioseparation, 6, 897 (1997)

[20] Miyata T, Asami N, Uragami T, Macromolecules, 32(6), 2082 (1999)

[21] Schmidt AM, Colloid Polym. Sci., 285, 953 (2007)

[22] Sauzedde F, Ela ssari A, Pichot C, Colloid Polym. Sci., 277, 1041 (1999)

[23] Cai J, Guo J, Ji M, Yang W, Wang C, Fu S, Colloid Polym. Sci., 285, 1607 (2007)

[24] Zhu Y, Wu Q, J. Nanopart. Res., 1, 393 (1999)

[25] Tang BZ, Geng Y, Lam JWY, Li B, Jing X, Wang X, Pakhomov AB, Zhang XX, Chem. Mater., 11, 1581 (1999)

[26] Ahmad H, Rahman MS, Miah MAJ, Ali AMI, Colloid Polym. Sci., 279, 1039 (2001)

[27] Zhang JL, Srivastava RS, Misra RDK, Langmuir, 23(11), 6342 (2007)

[28] Ma M, Zhang Y, Yu W, Shen HY, Zhang HQ, Gu N, Colloid Surface A, 212, 219 (2003)

[29] Sayar F, Guven G, Pi kin E, Colloid Polym. Sci., 284, 965 (2006)

[30] Okubo M, Ahmad H, Colloid Polym. Sci., 273, 817 (1995)