Production and structural features of a water-soluble polysaccharide from a mutant strain of Agrobacterium sp.

Shinsook Yoon; Mi-Kyoung Kim; In-Young Lee; Mikyung Yun; Jeong E. Nam Shin

Journal of Industrial and Engineering Chemistry, Vol.14, No.6, 759-764, November, 2008

DOI10.1016/j.jiec.2008.06.001 Export Citation

Production and structural features of a water-soluble polysaccharide from a mutant strain of Agrobacterium sp.

Shinsook Yoon^†, Mi-Kyoung Kim¹, In-Young Lee¹, Mikyung Yun², and Jeong E. Nam Shin³

College of General Education, Kangnam University, Yongin 449-702, Republic of Korea
¹DMJ Biotech Corp., Yeongi-gun, Chungcheongnam-do 339-824, Republic of Korea
²Department of Applied Chemistry, Dongyang Technical College, Seoul 152-714, Republic of Korea
³Department of Chemistry, Soongsil University, Seoul 156-743, Republic of Korea

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We have developed a mutant strain derived from Agrobacterium sp. ATCC 31750, which produces a water-soluble polysaccharide having potential utility to the food, feed, pharmaceutical and cosmetic industries. A high concentration of product (15 g/L) is obtained by 48 h cultivation of the mutant strain under optimized fermentation conditions. The water-soluble polysaccharide obtained from cultures of the mutant strain beta82 has Glc:Man:Gal in approximate molar ratios of 5.8:6.7:1.0. The molecular weight of the polysaccharide was determined to be approximately 1000 kDa by HPSEC analysis. Linkage analysis contained 3-Glcp, 3-Manp, terminal Glcp and terminal Manp, as well as a small proportion of 3- and 3,4-Galp, and 4,6-Manp residues. Based on analyses using FT-IR and 13C NMR spectrometers, most glycosidic bonds joining these sugar residues are of the α-type, and acetyl groups are apparently attached to the polymer chain at random.

Keywords:Water-soluble polysaccharide;Agrobacterium sp.;Mutant strain;Production;Structure

[References]

Kim MK, Ryu KE, Choi WA, Rhee YH, Lee IY, Biochem. Eng. J., 16, 163 (2003)
McKellar RC, van Geest J, Cui W, Food Hydrocolloids, 17, 429 (2003)
Kim YH, Kang SW, Lee JH, Chang HI, Yun CW, Paik HD, Kang CW, Kim SW, J. Ind. Eng. Chem., 13(1), 153 (2007)
Kamisato JK, Nowakowski M, Immunopharmacology, 16, 88 (1988)
Jong SC, Birmingham JM, Adv. Appl. Microbiol., 39, 153 (1993)
Numasaki Y, Kikuchi M, Sugiyama Y, Ohba Y, Oyo Yakuri Pharmacometrics, 39, 39 (1990)
Sandula J, Machova E, Hribalova V, Int. J. Biol. Macromol., 17, 323 (1995)
Amemura A, Hisamatsu M, Harada T, Appl. Environ. Microbiol., 34, 617 (1977)
Korea Patent No. 2003-17803
Harada T, Arch. Biochem. Biophys., 112, 65 (1965)
Miller GL, Anal. Chem., 31, 426 (1972)
Sriene F, Arnold B, Bailey JE, Biotechnol. Bioeng., 26, 982 (1984)
Ciucanu I, Ker F, Carbohydr. Res., 131, 209 (1984)
Read SM, Currie G, Bacic A, Carbohydr. Res., 281, 187 (1995)
Naganishi I, Kimura K, Kusui S, Yamazaki E, Carbohydr. Res., 32, 47 (1974)
Lee IY, Seo WT, Kim GJ, Kim MK, Park CS, Park YH, J. Ind. Microbiol. Biotechnol., 18, 255 (1997)
Kim MK, Lee IY, Lee JH, Rhee YH, Park YH, J. Ind. Microbiol. Biotechnol., 25, 180 (2000)
Lee JH, Lee IY, Kim MK, Park YH, J. Ind. Microbiol. Biotechnol., 23, 143 (1999)
Lee IY, Kim MK, Lee JH, Seo WT, Jung JK, Lee HW, Park YH, Bioprocess Eng., 20, 283 (1999)
Harada T, Agric. Biol. Chem., 34, 1683 (1970)
Cote GL, Ahlgren JA, Smith MR, J. Ind. Microbiol. Biotechnol., 23, 656 (1999)
Ukai S, Hirose K, Kiho T, Hara C, Chem. Pharm. Bull., 22, 1102 (1974)
Kath F, Kulicke WM, Angew. Makromole. Chem., 268, 69 (1999)
Senchenkova SN, Shashkov AS, Knirel YA, McGovern JJ, Moran AP, Eur. J. Biochem., 245, 637 (1997)
Mizuno M, Morimoto M, Minato K, Tsuchida H, Biosci. Biotechnol. Biochem., 62, 434 (1998)
Pramanik M, Mondal S, Chakraborty I, Rout D, Islam SS, Carbohydr. Res., 340, 629 (2005)
Jin Y, Zhang L, Zhang M, Chen L, Cheung PCK, Oi VEC, Lin Y, Carbohydr. Res., 338, 1517 (2003)

[Referenced By]

[1] Kim MK, Ryu KE, Choi WA, Rhee YH, Lee IY, Biochem. Eng. J., 16, 163 (2003)

[2] McKellar RC, van Geest J, Cui W, Food Hydrocolloids, 17, 429 (2003)

[3] Kim YH, Kang SW, Lee JH, Chang HI, Yun CW, Paik HD, Kang CW, Kim SW, J. Ind. Eng. Chem., 13(1), 153 (2007)

[4] Kamisato JK, Nowakowski M, Immunopharmacology, 16, 88 (1988)

[5] Jong SC, Birmingham JM, Adv. Appl. Microbiol., 39, 153 (1993)

[6] Numasaki Y, Kikuchi M, Sugiyama Y, Ohba Y, Oyo Yakuri Pharmacometrics, 39, 39 (1990)

[7] Sandula J, Machova E, Hribalova V, Int. J. Biol. Macromol., 17, 323 (1995)

[8] Amemura A, Hisamatsu M, Harada T, Appl. Environ. Microbiol., 34, 617 (1977)

[9] Korea Patent No. 2003-17803

[10] Harada T, Arch. Biochem. Biophys., 112, 65 (1965)

[11] Miller GL, Anal. Chem., 31, 426 (1972)

[12] Sriene F, Arnold B, Bailey JE, Biotechnol. Bioeng., 26, 982 (1984)

[13] Ciucanu I, Ker F, Carbohydr. Res., 131, 209 (1984)

[14] Read SM, Currie G, Bacic A, Carbohydr. Res., 281, 187 (1995)

[15] Naganishi I, Kimura K, Kusui S, Yamazaki E, Carbohydr. Res., 32, 47 (1974)

[16] Lee IY, Seo WT, Kim GJ, Kim MK, Park CS, Park YH, J. Ind. Microbiol. Biotechnol., 18, 255 (1997)

[17] Kim MK, Lee IY, Lee JH, Rhee YH, Park YH, J. Ind. Microbiol. Biotechnol., 25, 180 (2000)

[18] Lee JH, Lee IY, Kim MK, Park YH, J. Ind. Microbiol. Biotechnol., 23, 143 (1999)

[19] Lee IY, Kim MK, Lee JH, Seo WT, Jung JK, Lee HW, Park YH, Bioprocess Eng., 20, 283 (1999)

[20] Harada T, Agric. Biol. Chem., 34, 1683 (1970)

[21] Cote GL, Ahlgren JA, Smith MR, J. Ind. Microbiol. Biotechnol., 23, 656 (1999)

[22] Ukai S, Hirose K, Kiho T, Hara C, Chem. Pharm. Bull., 22, 1102 (1974)

[23] Kath F, Kulicke WM, Angew. Makromole. Chem., 268, 69 (1999)

[24] Senchenkova SN, Shashkov AS, Knirel YA, McGovern JJ, Moran AP, Eur. J. Biochem., 245, 637 (1997)

[25] Mizuno M, Morimoto M, Minato K, Tsuchida H, Biosci. Biotechnol. Biochem., 62, 434 (1998)

[26] Pramanik M, Mondal S, Chakraborty I, Rout D, Islam SS, Carbohydr. Res., 340, 629 (2005)

[27] Jin Y, Zhang L, Zhang M, Chen L, Cheung PCK, Oi VEC, Lin Y, Carbohydr. Res., 338, 1517 (2003)