Fabrication of Multicomponent Protein Microarrays with Microfluidic Devices of Poly(dimethylsiloxane)

Sehoon Jeon; Ui Seong Kim; Wonjin Jeon; Chee Burm Shin; Surin Hong; Inhee Choi; Suseung Lee; Jongheop Yi

Macromolecular Research, Vol.17, No.3, 192-196, March, 2009

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Fabrication of Multicomponent Protein Microarrays with Microfluidic Devices of Poly(dimethylsiloxane)

Sehoon Jeon, Ui Seong Kim, Wonjin Jeon, Chee Burm Shin^†, Surin Hong¹, Inhee Choi¹, Suseung Lee¹, and Jongheop Yi¹

Division of Energy Systems Research, Ajou University, Suwon 443-749, Korea
¹School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Korea

E-mail:

Recently, the multi-screening of target materials has been made possible by the development of the surface plasmon resonance (SPR) imaging method. To adapt this method to biochemical analysis, the multi-patterning technology of protein microarrays is required. Among the different methods of fabricating protein microarrays, the microfluidic platform was selected due to its various advantages over other techniques. Microfluidic devices were designed and fabricated with polydimethylsiloxane (PDMS) by the replica molding method. These devices were designed to operate using only capillary force, without the need for additional flow control equipment. With these devices, multiple protein-patterned sensor surfaces were made, to support the two-dimensional detection of various protein-protein interactions with SPR. The fabrication technique of protein microarrays can be applied not only to SPR imaging, but also to other biochemical analyses.

Keywords:protein microarray;microfluidic device;polydimethylsiloxane.

[References]

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[Referenced By]

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[3] Homola J, Yee SS, Gauglitz G, Sensor Actuat. BChem., 54, 3 (1999)

[4] Green RJ, Frazier RA, Shakeshe KM, Davies MC, Roberts CJ, Tendler SJB, Biomaterials, 21, 1823 (2000)

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[10] Gerlach A, Knebel G, Guber AE, Heckele M, Herrmann D, Muslija A, Schaller T, Microsyst. Technol., 7, 265 (2002)

[11] Cunningham DD, Analy. Chim. Acta, 429, 1 (2001)

[12] Weigl BH, Hedine K, American Clinical Laboratory, 21, 8 (2002)

[13] Reyes DR, Iossifidis D, Auroux P, Manz A, Anal. Chem., 74, 2623 (2002)

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[15] Nguyen N, Wereley ST, Fundamentals and Applications of Microfluidics, 2nd Ed., Artech House, Norwood, MA (2006)

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[19] Mills CA, Martinez E, Bessueille F, Villanueva G, Bausells J, Samitier J, Errachid A, Microelectronic Engineering, 78, 695 (2005)

[20] Metz S, Holzer R, Renaud P, Lab Chip, 1, 29 (2001)

[21] Duffy DC, McDonald JC, Schueller OJA, Whitesides GM, Anal. Chem., 70, 4974 (1998)

[22] Urban G, Ed., BioMEMS, Springer, Dordrecht, Netherlands (2006)

[23] Wheeler AR, Chah S, Whelan RJ, Zare RN, Sensor Actuat. B-Chem., 98, 208 (2004)

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[27] Al-Chalabi A, Leigh PN, Curr. Opin. Neurol., 13, 397 (2000)

[28] Shoesmith CL, Strong MJ, Can. Fam. Physician, 52, 1563 (2006)

[29] Al-Chalabi A, Leigh PN, Curr. Opin. Neurol., 13, 397 (2000)

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[31] Schmidt PJ, Kunst C, Culotta VC, J. Biol. Chem., 275, 33771 (2000)