화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of the Korean Industrial and Engineering Chemistry, Vol.20, No.3, 290-295, June, 2009
Export Citation
Saccharomyces cerevisiae 에 의한 Agar로부터 바이오 에탄올 생산
Production of Bio-ethanol from Agar using Saccharomyces cerevisiae
이성목, 유병조1, 김영민, 최수정, 하종명, 이재화†
  • 신라대학교 공과대학 생명공학과
  • 1삼성종합기술원
Sung-Mok Lee, Byung Jo Yu1, Young Min Kim, Soo-Jeong Choi, Jong-Myung Ha, and Jae-Hwa Lee†
  • Department of Bioscience and Biotechnology, College of Engineering, Silla University, Busan 617-736, Korea
  • 1Bio & Health Group, Emerging Center, Samsung Advanced Institute of Technology, Kyunggi-Do 446-712, Korea
E-mail:
초록
해조류 중에서도 홍조류의 agar는 D-galactose와 3,6-anhydro-L-galactose로 구성되어 있기 때문에 이를 분해하면 바이오 에탄올을 생산 할 수 있는 가능성이 높다. 본 연구에서는 열처리와 산 처리를 이용하여 agar를 당화하고 이를 통해 바이오 에탄올을 생산하고자 한다. 바이오 에탄올을 생산하기 위하여 전처리 된 agar에 Saccharomyces cerevisiae KCCM1129를 접종하여 발효하였다. Agar로부터 환원당 생성의 최적조건은 0.1 N HCl이었고, 120 ℃에서 15 min 반응하는 것으로 확인되었다. 발효균주 성장을 위한 최적 염 농도는 0.1 N NaCl로 17.88 g/L까지 성장하였으며, 0.1 N 이상의 농도에서 6.78∼10.76 g/L로 성장이 감소했다. 그리고 agar 16% 농도에서 최적 전처리에 의한 에탄올 생산은 10.16 g/L이었다.
Red-algae agar, consisting of D-galactose and 3, 6-anhydro-L-galactose, is usable for bio-ethanol production if hydrolyzed to monomer unit. The objective of this study is to produce bio-ethanol from agar using the heat and acid-treatment. Bio-ethanol was produced by Saccharomyces cerevisiae KCCM1129 strains using agar-pretreatment. The optimal condition for reducing sugar conversion by agar was found to be 15 min reaction at a HCl concentration of 0.1 N and 120 ℃. The optimum concentration for maximum cell growth was 0.1 N NaCl (17.88 g/L). Over 0.1 N NaCl, the cell growth decreased to 6.78∼10.76 g/L. At 16% agar concentration, the ethanol production obtained by optimum pretreatment was found to be 10.16 g/L.
Keywords:Saccharomyces cerevisiae;sea weed;bio-ethanol;agar;saccharification
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