화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.26, No.5, 1365-1372, September, 2009
DOI10.1007/s11814-009-0211-x  Export Citation
Salt-induced protein separation in an aqueous electrolyte solution
Sang Ha Choi, and Young Chan Bae†
  • Division of Chemical Engineering and Molecular Thermodynamics Laboratory, Hanyang University, Seoul 133-791, Korea
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Liquid-liquid phase separations of aqueous ovalbumin and bovine serum albumin (BSA) solutions are reported experimentally for a wide range of solution conditions. The temperature-induced clouding of protein solutions, which signals the onset of liquid-liquid phase separation, provides a simple means of assessing the effect of solution conditions on the strength of protein interaction. Our results show that the effect of salts on protein interactions depends sensitively on the ionic composition of solution and the identities of both the cation and the anion of the added salts. The results are used to test and refine theoretical models for the interaction energy between macromolecules. A modified perturbed hard-sphere chain (MPHSC) model is employed to determine the interaction energy for solvation forces playing an important role in protein interactions and to predict the osmotic pressures of protein solutions.
Keywords:BSA;Ovalbumin;Liquid-liquid Phase Separation;Cloud-point Temperature (CPT);MPHSC
  1. Blanch HW, Prausnitz JM, Curtis RA, Bratko D, Fluid Phase Equilib., 194, 31 (2002)
  2. Tavares FW, Bratko D, Striolo A, Blanch HW, Prausnitz JM, J. Chem. Phys., 120, 20 (2004)
  3. Haas C, Drenth J, J. Phys. Chem. B, 102(21), 4226 (1998)
  4. Scopes RK, Protein purification: Principles and practice, Springer-Verlag, New York Inc. (1982)
  5. Asherie N, Lomakin A, Benedek GB, Phys. Rev. Lett., 77, 4832 (1996)
  6. Broide ML, Tominc TM, Saxowsky MD, Phys. Rev. E, 6 (1995)
  7. Kim SG, Kong SH, Bae YC, Kim S, Macromol. Res., 11(4), 241 (2003)
  8. Flory P, Principles of polymer chemistry, Cornell University Press, Ithaca (1971)
  9. Gelbart WM, Ben-Shaul A, Roux D, Michelles, membranes, microemulsions, and monolayers, Springer-Verlag, New York (1994)
  10. Tenwolde PR, Frenkel D, Science, 277(5334), 1975 (1997)
  11. Rupert LAM, J. Colloid Int. Sci., 153, 92 (1992)
  12. Rothstein F, In protein precipitation process engineering, Harrion RG, Ed., Dekker, New York (1994)
  13. Kim SG, Bae YC, Macromol. Res., 11(1), 53 (2003)
  14. Kuehner DE, Blanch HW, Prausnitz JM, Fluid Phase Equilib., 116(1-2), 140 (1996)
  15. Fornasiero F, Ulrich J, Prausnitz JM, Chem. Eng. Process., 38(4-6), 463 (1999)
  16. Jiang JW, Prausnitz JM, J. Phys. Chem. B, 103(26), 5560 (1999)
  17. Liu W, Bratko D, Prausnitz JM, Blanch HW, Biophys. Chem., 107, 289 (2004)
  18. Grigsby JJ, Blanch HW, Prausnitz JM, J. Phys. Chem. B, 104(15), 3645 (2000)
  19. Malfois M, Bonnete F, Belloni L, Tardieu A, J. Chem. Phys., 105(8), 3290 (1996)
  20. Curtis RA, Ulrich J, Montaser A, Prausnitz JM, Blanch HW, Biotech. Bioeng., 79, 4 (2002)
  21. Curtis RA, Prausnitz JM, Blanch HW, Biotech. Bioeng., 57, 1 (1998)
  22. Gedde UW, Polymer physics, Chapman and Hall (1995)
  23. Park EJ, Bae YC, Biophys. Chem., 109, 169 (2004)
  24. Malcolm GN, Rowlinson JS, Trans. Faraday Soc., 53, 921 (1967)
  25. Mackenzie RC, Thermochimica Acta, 28, 1 (1979)
  26. Mackenzie RC, Talanta, 16, 1227 (1969)
  27. Mackenzie RC, Keattch CJ, Dollimore D, Forrester JA, Hodgson AA, Redfern JP, Talanta, 19, 1079 (1972)
  28. Prausnitz JM, Lichtenthaler RN, Azevedo EGD, Molecular thermodynamics of fluid-phase equilibria, 3rd Ed., Prentice-Hall PTR (1999)
  29. Walas SM, Phase equilibria in chemical engineering, Butterworths, Boston, MA (1985)
  30. Shen S, Lu BCY, Fluid Phase Equilib., 84, 9 (1993)
  31. Choi SH, Bae YC, Physica. A., submitted. (2005)
  32. Cousin F, Cabuil V, J. Mole. Liquid, 83, 203 (1999)
  33. McCarty BW, Adams ET, J. Biophys. Chem., 28, 149 (1987)
  34. Minton AP, Biophys. Chem., 57, 65 (1995)
  35. Yousef MA, Datta R, Rodgers VGJ, J. Colloid Interface Sci., 197(1), 108 (1998)
  36. Yousef MA, Datta R, Rodgers VGJ, J. Colloid Interface Sci., 207(2), 273 (1998)
  37. Yousef MA, Datta R, Rodgers VGJ, J. Colloid Interface Sci., 243(2), 321 (2001)
  38. Amos DA, Markels JH, Lynn S, Radke CJ, J. Phys. Chem. B, 102(15), 2739 (1998)
  39. Zimmerman RJ, Kanal KM, Sanders J, Cameron IL, Fullerton GD, J. Biochem. Biophys. Methods, 30, 113 (1995)
  40. Neal BL, Asthagiri D, Velev OD, Lenhoff AM, Kaler EW, J. Cryst. Growth, 196, 377 (1999)
  41. Moon YU, Anderson CO, Blanch HW, Prausnitz JM, Fluid Phase Equilib., 168, 299 (2000)
  42. Curtis RA, Steinbrecher C, Heinemann M, Blanch HW, Prausnitz JM, Biophys. Chem., 98, 249 (2002)
  43. McMillan Jr. WG, Mayer JE, J. Chem. Phys., 13, 276 (1945)
  44. Wu JZ, Prausnitz JM, Fluid Phase Equilib., 155(1), 139 (1999)
  45. Neal BL, Lenhoff AM, AIChE J., 41(4), 1010 (1995)
  46. Kuehner DE, Blanch HW, Heyer C, Ramsch C, Fornefeld UM, Prausnitz JM, Biophys. J., 73, 3211 (1997)
  47. Stein PE, Leslie AGW, Finch JT, Turnell WG, McLaughlin PJ, Carrell RW, Nature, 347, 99 (1990)
  48. Vilker VL, Colton CK, Smith KA, J. Colloid Int. Sci., 79, 548 (1981)
  49. Edsall JT, Proteins as acids and bases, in: Cohn EJ, Edsall JT, Eds., Proteins, Amino acids and Peptides, Reinhold Publishing Corp., New York (1943)
  50. Grigsby JJ, Blanch HW, Prausnitz JM, Biophys. Chem., 91, 231 (2001)
  51. Taratuta VG, Holschbach A, Thurston GM, J. Phys. Chem., 94, 2140 (1990)