화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.17, No.9, 688-696, September, 2009
Export Citation
Electrospun Antimicrobial Polyurethane Nanofibers Containing Silver Nanoparticles for Biotechnological Applications
Faheem A. Sheikh, Nasser A. M. Barakat1, †, Muzafar A. Kanjwal2, Atul A. Chaudhari3, In-Hee Jung3, John Hwa Lee3, and Hak Yong Kim4, †
  • Department of Bionano System Engineering, Chonbuk National University, Jeonju 561-756, Korea
  • 1Chemical Engineering Department, Faculty of Engineering, El-Minia University, El-Minia, EgyptCenter for Healthcare Technology Development, Chonbuk National University, Jeonju 561-756, Korea
  • 2Department of Polymer Nano Science and Technology, Chonbuk National University, Jeonju 561-756, Korea
  • 3College of Veterinary Medicine, Chonbuk National University, Jeonju 561-756, Korea
  • 4Department of Textile Engineering, Chonbuk National University, Jeonju 561-756, Korea
E-mail:,
In this study, a new class of polyurethane (PU) nanofibers containing silver (Ag) nanoparticles (NPs) was synthesized by electrospinning. A simple method that did not depending on additional foreign chemicals was used to self synthesize the silver NPs in/on PU nanofibers. The synthesis of silver NPs was carried out by exploiting the reduction ability of N,N-dimethylformamide (DMF), which is used mainly to decompose silver nitrate to silver NPs. Typically, a sol-gel consisting of AgNO3/PU was electrospun and aged for one week. Silver NPs were created in/on PU nanofibers. SEM confirmed the well oriented nanofibers and good dispersion of pure silver NPs. TEM indicated that the Ag NPs were 5 to 20 nm in diameter. XRD demonstrated the good crystalline features of silver metal. The mechanical properties of the nanofiber mats showed improvement with increasing silver NPs content. The fixedness of the silver NPs obtained on PU nanofibers was examined by harsh successive washing of the as-prepared mats using a large amount of water. The results confirmed the good stability of the synthesized nanofiber mats. Two model organisms, E. coli and S. typhimurium, were used to check the antimicrobial influence of these nanofiber mats. Subsequently, antimicrobial tests indicated that the prepared nanofibers have a high bactericidal effect. Accordingly, these results highlight the potential use of these nanofiber mats as antimicrobial agents.
Keywords:electrospinning;nanofibers;silver nanoparticles;antimicrobial;zones of inhibition;morphological changes
  1. Peter AL, Adv. Drug Deliver. Rev., 57, 1471 (2005)
  2. Berger TJ, Spadaro JA, Chapin SE, Becker RO, Antimicrob. Agents Chemother., 9, 357 (1976)
  3. Silver S, Fems Microbiol. Rev., 27, 341 (2003)
  4. Nair LS, Laurencin CT, J. Biomed. Nanotech., 3, 16 (2007)
  5. Tian J, Wong KK, Ho CM, Lok CN, Yu WY, Che CM, Chiu JF, Tam PK, Chem. Med. Chem., 2, 129 (2007)
  6. Trogolo J, Filtr. Sep., 43, 28 (2006)
  7. Innes ME, Umraw N, Fish JS, Gomez M, Cartotto RC, Burns, 27, 621 (2001)
  8. Butkus MA, Talbot M, Labare MP, Water Res., 39, 4925 (2005)
  9. DeBono RF, Helluy A, Heimlich M, Krull UJ, Sens. Actuators, B, 11, 487 (1993)
  10. Gibson PW, Schreuder-Gibson HL, Rivin D, AIChE J., 45(1), 190 (1999)
  11. Gibson PW, Schreuder-Gibson HL, Rivin D, Colloid Surf. A, 187/188, 469 (2001)
  12. Son WK, Youk JH, Park WH, Carbohydr. Polym., 65, 430 (2006)
  13. Lee HK, Jeong EH, Chi Baek CK, Youk JH, Mater. Lett., 59, 2977 (2005)
  14. Duan YY, Jia J, Wang SH, Yan W, Jin L, Wang ZY, J. Appl. Polym. Sci., 106(2), 1208 (2007)
  15. Kong H, Jang J, Langmuir, 24(5), 2051 (2008)
  16. Hong KH, Park JL, Sul IH, Youk JH, Kang TJ, J. Polym. Sci. B: Polym. Phys., 44(17), 2468 (2006)
  17. Zhang Q, Wu D, Qi S, Wu Z, Yang X, Jin R, Mater. Lett., 61, 4027 (2007)
  18. Jung YH, Kim HY, Lee DR, Park SY, Khil MS, Macromol. Res., 13(5), 385 (2005)
  19. Doshi J, Reneker DH, J. Electrostat., 35, 151 (1995)
  20. Jin HJ, Hwang MO, Yoon JS, Lee KH, Chin IJ, Kim MN, Macromol. Res., 13(1), 73 (2005)
  21. Barnes CP, Sell SA, Boland ED, Simpson DG, Bowlin GL, Adv. Drug Deliver. Rev., 59, 1413 (2007)
  22. Aussawasathien D, Teerawattananon C, Vongachariya A, J. Membr. Sci., 315(1-2), 11 (2008)
  23. Kidoaki S, Kwon IK, Matsuda T, J. Biomed. Mater. Res. Part B: Appl. Biomater., 786, 219 (2006)
  24. Khlystalova TK, Kurganova MN, Demina AI, Petova MB, Tarakanov OG, Mech. Compos. Mater., 21, 763 (1986)
  25. Han JH, Taylor JD, Kim DS, Kim YS, Kim YT, Cha GS, Nam H, Sens. Actuators B, 123, 384 (2007)
  26. Hains N, Friscic V, Gordos D, Int. J. Cloth. Sci. Technol., 15, 250 (2003)
  27. Khil MS, Cha DI, Kim HY, Kim IS, Bhattarai N, J. Biomed. Mater. Res. B: Appl. Biomater., 67, 657 (2003)
  28. Yao C, Li X, Neoh KG, Shi Z, Kang ET, J. Membr. Sci., 320, 295 (2008)
  29. Jeong EH, Yang J, Youk JH, Mater. Lett., 61, 3991 (2007)
  30. Pastoriza-Santos I, Liz-Marzan LM, Pure Appl. Chem., 72, 83 (2000)
  31. Pastoriza-Santos I, Liz-Marzan LM, Langmuir, 15, 849 (1999)
  32. Lala NL, Ramaseshan R, Li BJ, Sundarrajan S, Barhate RS, Liu YJ, Ramakrishna S, Biotechnol. Bioeng., 97(6), 1357 (2007)
  33. Zhuo HT, Hu JH, Chen SJ, Yeung LP, J. Appl. Polym. Sci., 109(1), 406 (2008)
  34. Guelcher SA, Tissue Eng. Part B, 14, 3 (2008)
  35. Park JH, Kim BS, Yoo YC, Khil MS, Kim HY, J. Appl. Polym. Sci., 107(4), 2211 (2008)
  36. Kim KW, Effects of Electrospinning Parameters on the Fiber Formation, PhD theses, Chonbuk Nat. Univ. (2007), and reference therein.
  37. Bohrem CF, Huffman DR, Absorption and Scattering of Light by Small Particles, Wiley-Interscience, New York (1983)
  38. Nikolajsen T, Leosson K, Bozhevolnyi SI, Appl. Phys. Lett., 85, 5833 (2004)
  39. Haynes CL, Van Duyne RP, J. Phys. Chem. B, 107(30), 7426 (2003)
  40. Hodak JH, Martini I, Hartland GV, J. Phys. Chem. B, 102(36), 6958 (1998)
  41. Patel K, Kapoor S, Dave DP, Mukerjee T, J. Chem. Sci., 117, 53 (2005)
  42. Atiyeh BS, Costagliola M, Hayek SN, Dibo SA, Burns, 33, 139 (2007)
  43. Gallant-Behm CL, Yin HQ, Liu S, Heggers JP, Langford RE, Olson ME, Hart DA, Burrell RE, Wound Repair Regen., 13, 412 (2005)
  44. Cho KH, Park JE, Osaka T, Park SG, Electrochim. Acta, 51(5), 956 (2005)