A sample preparation method based on convective assembly for "whole-microorganism" identification using surface-enhanced Raman scattering (SERS) is developed. With this technique, a uniform sample can easily be prepared with silver nanoparticles. During the deposition process, bacteria and nanoparticles are assembled to form a unique well-ordered structure with great reproducibility. The SERS spectra acquired from the samples prepared with this technique have better quality and improved reproducibility for SERS spectra obtained from the same sample and limited variation due to the consistent sample preparation. E. coli, a Gram-negative bacilli, and Staphylococcus cohnii, a Gram-positive coccus, are studied as model bacteria.