Two strains isolated from the organic layers of forests on Zijin Mountain have indicated a strong capability of decolorization for Orange G on the solid plates. They were identified as Phanerochaete sordida and Tyromyces lauteus according to phenotypic and molecular techniques. Through this study, we try to find the suitable condition and cheapest way for decolorization by two strains. The result shows that malt extract and ammonium sulfate are the best N source for P. sordida and T lauteus, respectively; 0.95 g per L glucose + 0.05 g per L ethonal are the best C source both for P. sordida and T lauteus. Oxalate plays an important role as the organic acid chelator which can also enhance the decolorized capability of fungi. (C) 2007 Elsevier Ltd. All rights reserved.