측쇄에 에스테르기를 갖는 폴리우레탄의 표면개질 및 혈액적합성

최명석; 강인규; 김교한; 이영무; 성용길

Polymer(Korea), Vol.21, No.5, 709-717, September, 1997

Export Citation

측쇄에 에스테르기를 갖는 폴리우레탄의 표면개질 및 혈액적합성

Surface Modifications of Polyurethane Containing Ester Groups in the Side Chain and Their Blood Compatibility

최명석, 강인규, 김교한, 이영무, 성용길

초록

폴리테트라메틸렌글리콜 (PTMG)과 메틸렌디페닐디이소시아네이트 (MDI)로부터 폴리우레탄 prepolymer를 합성한 다음 에틸디히드록시메틸프로피조네이트 (EDHMP)로 사슬연장하여 폴리에테르우레탄(PU)을 합성하였다. PU필름을 화학적으로 개질하여 표면에 카르복시기 (PY-C), 폴리에틸렌옥시드(PU-PEO), 및 혜파린 (PU-C-Hep, PY-PEO-Hep)을 도입하였으며 이들의 invitro 혈액적합성을 조사하였다. 헤파린이 고정화된 PU-C-Hep와 PU-PEO-Hep의 경우 1시간 동안 혈액과 접촉하였을 때 생성된 혈전이 각각 38%, 35%로 가장 적었다. 또한 혜파린 고정화 요면에서는 칼슘재첨가 응고시간(PRT)과 부분활성화 트롬보플라스틴 시간(APTT)이 다른 표면보다 길게 나타나 혈장단백질의 활성차가 억제됨을 알 수 있었다 한편 헤파린 고정화 표면에 점착한 혈소판의 양은 다른 표면에서보다 강대적으로 적은 값을 나타내었고, 이들 결과는 주사형 전자현미경으로 관찰한 결과와 일치하였다.

Polyetherurethane (PU) was synthesized by reaction of 4,4'-diphenylmethane diisocyanate (MDI) and polytetramethylene glycol (PTMG) followed by chain extention reaction with ethyl 2,2-dihydroxymethylpropionate (EDHMP). PU films were chemically modified to obtain carboxylic acid-introduced PU (PU-C), polyoxyethylene-grafted PU (PU-PEO) and heparin-immobilized PU (PU-C-Hep, PU-PEO-Hep) and their in-vitro blood compatibility were examined. PU-C-Hep (38%) and PU-PEO-Hep (35%) showed relatively low thrombus formation when they contacted with blood for 1 h. Plasma recalcification time (PRT) and activated partial thromboplastin time (APTT) were increased by heparin immobilization, showing the suppression of the activation of plasma proteins. These results were consistent with those obtained by the observation of scanning electron microscope (SEM).

Keywords:polyertherurethane;EDHMP;surface modification;in-vitro bolld compatibility

[References]

Iwata K, "Handbook of Polyurethane Resin," Nikankokyo Shinbunsha, p. 620 (1987)
Szycher M, "High Performance Biomaterials," Technomic Publishing Company, Inc. (1991)
Han MJ, Kwon YH, Polym.(Korea), 2(4), 204 (1978)
Ahn TO, Lee SY, Lee SW, Jeong HM, Polym.(Korea), 14(5), 497 (1990)
Imanishi Y, "Biomedical Polymers," Kyoritsu Shupan, p. 19 (1986)
Ito Y, Iguchi Y, Imanishi Y, Biomaterials, 13, 131 (1992)
Yui N, Sanui K, Ogata N, Kataoka K, Okano T, Sakurai Y, J. Biomed. Mater. Res., 20, 929 (1986)
Shibuta R, Tanaka M, Sisido M, Imanishi Y, J. Biomed. Mater. Res., 20, 971 (1986)
Ito Y, Sisido M, Imanishi Y, J. Biomed. Mater. Res., 20, 1139 (1986)
Park KD, Okano T, Nojiri C, Kim SW, J. Biomed. Mater. Res., 22, 977 (1988)
Han DK, Park KD, Ahn KD, Jeong SY, Kim YH, J. Biomed. Mater. Res.: Appl. Biomater., 23, 87 (1989)
Han DK, Jeong SY, Kim YH, J. Biomed. Mater. Res. Appl. Biomater., 23, 211 (1989)
Ito Y, Sisido M, Imanishi Y, J. Biomed. Mater. Res., 20, 1157 (1986)
Kang IK, Kwon OH, Kim MK, Lee YM, Sung YK, Biomaterials, 18, 1099 (1997)
Sinser II, Kawka DW, Scott S, Munford RA, J. Cell. Biol., 104, 573 (1987)
Moore S, J. Biol. Chem., 243, 281 (1968)
Smith PK, Mallia AK, Harmanson GT, Anal. Biochem., 109, 466 (1980)
Imai Y, Nose Y, J. Biomed. Mater. Res., 6, 165 (1972)
Ito Y, Iwata K, Kang IK, Sisido M, Imanishi Y, Int. J. Biol. Macromol., 10, 169 (1988)
Lelah MD, Grasel TG, Pierce TA, Cooper SL, J. Biomed. Mater. Res., 20, 433 (1986)
Jung HW, Lee JH, Kang IK, Polym.(Korea), 19(1), 117 (1995)
Okano T, Nishiyama S, Shinohara I, Akaike T, Sakurai Y, Kataoka K, Tsuruta T, J. Biomed. Mater. Res., 35, 393 (1981)
Ko TM, Cooper SL, J. Appl. Polym. Sci., 47, 1601 (1993)
Kang IK, Kwon OH, Byun KH, Kim YH, J. Mater. Sci. Mater. Med., 7, 135 (1996)
Kang IK, Kwon OH, Lee YM, Sung YK, Biomaterials, 17, 841 (1996)
Kang IK, Kwon BK, Lee JH, Lee HB, Biomaterials, 14, 787 (1993)
Ito Y, Sisido M, Imanishi Y, J. Biomed. Mater. Res., 23, 191 (1989)
Ito Y, Sisido M, Imanishi Y, J. Biomed. Mater. Res., 24, 227 (1989)

[Referenced By]

[1] Iwata K, "Handbook of Polyurethane Resin," Nikankokyo Shinbunsha, p. 620 (1987)

[2] Szycher M, "High Performance Biomaterials," Technomic Publishing Company, Inc. (1991)

[3] Han MJ, Kwon YH, Polym.(Korea), 2(4), 204 (1978)

[4] Ahn TO, Lee SY, Lee SW, Jeong HM, Polym.(Korea), 14(5), 497 (1990)

[5] Imanishi Y, "Biomedical Polymers," Kyoritsu Shupan, p. 19 (1986)

[6] Ito Y, Iguchi Y, Imanishi Y, Biomaterials, 13, 131 (1992)

[7] Yui N, Sanui K, Ogata N, Kataoka K, Okano T, Sakurai Y, J. Biomed. Mater. Res., 20, 929 (1986)

[8] Shibuta R, Tanaka M, Sisido M, Imanishi Y, J. Biomed. Mater. Res., 20, 971 (1986)

[9] Ito Y, Sisido M, Imanishi Y, J. Biomed. Mater. Res., 20, 1139 (1986)

[10] Park KD, Okano T, Nojiri C, Kim SW, J. Biomed. Mater. Res., 22, 977 (1988)

[11] Han DK, Park KD, Ahn KD, Jeong SY, Kim YH, J. Biomed. Mater. Res.: Appl. Biomater., 23, 87 (1989)

[12] Han DK, Jeong SY, Kim YH, J. Biomed. Mater. Res. Appl. Biomater., 23, 211 (1989)

[13] Ito Y, Sisido M, Imanishi Y, J. Biomed. Mater. Res., 20, 1157 (1986)

[14] Kang IK, Kwon OH, Kim MK, Lee YM, Sung YK, Biomaterials, 18, 1099 (1997)

[15] Sinser II, Kawka DW, Scott S, Munford RA, J. Cell. Biol., 104, 573 (1987)

[16] Moore S, J. Biol. Chem., 243, 281 (1968)

[17] Smith PK, Mallia AK, Harmanson GT, Anal. Biochem., 109, 466 (1980)

[18] Imai Y, Nose Y, J. Biomed. Mater. Res., 6, 165 (1972)

[19] Ito Y, Iwata K, Kang IK, Sisido M, Imanishi Y, Int. J. Biol. Macromol., 10, 169 (1988)

[20] Lelah MD, Grasel TG, Pierce TA, Cooper SL, J. Biomed. Mater. Res., 20, 433 (1986)

[21] Jung HW, Lee JH, Kang IK, Polym.(Korea), 19(1), 117 (1995)

[22] Okano T, Nishiyama S, Shinohara I, Akaike T, Sakurai Y, Kataoka K, Tsuruta T, J. Biomed. Mater. Res., 35, 393 (1981)

[23] Ko TM, Cooper SL, J. Appl. Polym. Sci., 47, 1601 (1993)

[24] Kang IK, Kwon OH, Byun KH, Kim YH, J. Mater. Sci. Mater. Med., 7, 135 (1996)

[25] Kang IK, Kwon OH, Lee YM, Sung YK, Biomaterials, 17, 841 (1996)

[26] Kang IK, Kwon BK, Lee JH, Lee HB, Biomaterials, 14, 787 (1993)

[27] Ito Y, Sisido M, Imanishi Y, J. Biomed. Mater. Res., 23, 191 (1989)

[28] Ito Y, Sisido M, Imanishi Y, J. Biomed. Mater. Res., 24, 227 (1989)