Chemistry Letters, Vol.37, No.1, 52-53, 2008
Synthesis of staudinger-type molecular probe for catch-and-release purification of the binding protein for potassium isolespedezate, a leaf-closing substance of leguminous plant
We synthesized azide-containing photoaffinity probe 1 based on the structure of potassium isolespedezate. This probe can be used for catch-and-release-mechanism purification of binding protein for 1: photo-crosslinking with I gave azide-labeled receptor which can be captured by phosphane-linked gel matrix by the Staudinger ligation. After washing the gel, the caught binding protein can be released by the reductive cleavage Of disulfide bond in 1. This process can be used as a convenient method for the purification of binding protein for bioactive natural product.