It is noteworthy that the thickness of fixed aqueous layer (TFAL) of three-component hybrid liposomes (THL) composed of L-alpha-dimyristoylphosphatidylcholine (DMPC), polyoxyethylene (20) sorbitan monolaurate (Tween 20), and decyl- -lactopranoside (LactC(10)) (DMPC:Tween 20:LactC(10) = 65:7:28) was about twice that of two-component hybrid liposomes (HL) composed of DMPC and Tween 20. It is also attractive that THL could remarkably inhibit the growth of hepatoma cells.