We present a nanowell (NW)-based biosensing platform for electrochemically detecting biological reactions on the level of single lipid vesicle. The NW geometry of a gently-sloped vertical wall is found to be optimal for selective deposition of single liposomes without capillary resistances. Also, the use of a hydrophilic, non-biofouling PEG copolymer is essential to provide distinctively separated FLV arrays without nonspecific adsorption.