We have reported an electro-active paper actuator from regenerated cellulose. After dissolving cellulose fibers with a solution of lithium chloride in N,N-dimethylacetamide, cellulose was regenerated by combining distillation of cellulose solution along with washing with the mixture of deionized water, isopropyl alcohol, and running water. However, the effect of Li+ ions on structure, properties, and the actuation behavior of the actuator was riot studied. This article describes the changes in these parameters when the Li+ ions are removed by subjecting it to different running water exposure time. The structure and properties of cellulose electro-active paper and its actuation behavior were studied. As Li+ ions content reduced from 4354.17 to 10.26 ppm by increasing the exposure time of running water, crystallinity, Young's modulus, and bending displacement decreased. Details about the investigation have been explained. This elimination of ions is important to increase the piezoelectric effect in EAPap by decreasing the ion migration effect. (C) 2008 Wiley Periodicals, Inc.