This research was conducted to evaluate the use of corn processing waste as an alternative growth medium for the cultivation of Lentinus edodes mycelium and to determine the optimum growth conditions under solid-state cultivation. The substrate concentration, pH, and temperature for maximizing the growth rate of L. edodes mycelium, 9.3 +/- 0.6 mm/d, were 44.3 g/l, 4.7, and 24.7 degrees C, respectively. Therefore, the results suggest that corn processing waste can be utilized as a growth substrate for cultivating L. edodes mycelium.