Vesicles have many important applications in many different fields. In the present paper, we report for the first time that oligonucleotide can induce single-chained cationic surfactant molecules to aggregate into vesicles by determining turbidity with a Uv-vis spectro photometer, observing images with a transmission electron microscope and/or fluorescence microscope, and dynamic light scattering. This study may increase the efficiency and applicability for a DNA/amphiphile system. (c) 2008 Elsevier Inc. All rights reserved.