화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.15, No.4, 520-523, July, 2009
DOI10.1016/j.jiec.2008.12.014  Export Citation
Colorimetric monitoring of the activity of recombinant Escherichia coli expressing styrene monooxygenase
Jong Wan Bae, Miso Park, Yong Joo Jeong1, Sunghoon Park†, and Sun-Gu Lee†
  • Department of Chemical and Biochemical Engineering, Pusan National University, Busan 609-735, Republic of Korea
  • 1Division of Nano Science, Kook Min University, Seoul 120-750, Republic of Korea
E-mail:,
Biocatalysis using recombinant Escherichia coli expressing styrene monooxygenase (SMO) activity is recognized as a promising process for the production of enantiopure aryl epoxides such as (S)-styrene oxide. Generally the activity of the whole cell biocatalyst is determined by measuring the conversion of styrene into (S)-styrene oxide by gas chromatography, which is however improper for the highthroughput analysis of many samples. Here we present that styrene monooxygenase catalyzed conversion of indole into indigo can be employed to monitor the activity of recombinant E. coli expressing styrene monooxygenase. We first confirmed that the colorimetric method was effective to monitor the whole cell activity of SMO by comparing the E. coli expressing styAB with that expressing only styB in a solid culture system. Next we used the monitoring method to investigate the effect of chaperone coexpression on the SMO whole cell activity in a liquid culture system.
Keywords:Colorimetric method;Styrene monooxygenase;Whole cell biocatalysis;Indole reaction
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