Flavobacterium sp. Strain DS5에 의한 Oleic Acid로부터 산화 지방산의 생산 및 회수

허신행; 김범수; Shin-Haeng Heo; Beom Soo Kim

Korean Chemical Engineering Research, Vol.47, No.5, 620-623, October, 2009

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Flavobacterium sp. Strain DS5에 의한 Oleic Acid로부터 산화 지방산의 생산 및 회수

Production and Recovery of Oxygenated Fatty Acids from Oleic Acid by Flavobacterium sp. Strain DS5

허신행, 김범수 ^†

충북대학교 화학공학과, 361-763 충북 청주시 흥덕구 성봉로 410

Shin-Haeng Heo, and Beom Soo Kim^†

Department of Chemical Engineering, College of Engineering, Chungbuk National University, 410 Seongbong-ro, Heungdeok-gu, Cheongju, Chungbuk 361-763, Korea

E-mail:

초록

Flavobacterium sp. strain DS5(NRRL B-14859)를 oleic acid로부터 중간생성물로서 10-hydroxystearic acid(10-HSA)를 거쳐 10-ketostearic acid(10-KSA)로의 전환에 이용하였다. 두 개의 플라스크 배양액을 원심분리하여 세포를 모은 후 한 개의 플라스크에 재현탁하여 세포농도를 증가시킴으로써 10-KSA의 생산이 보통 플라스크 배양의 3.5 g/L에서 6.5 g/L로 향상되었다. 배양액에 Tween-80의 첨가는 10-KSA와 10-HSA의 생산에 크게 영향을 미치지 않았다. 발효 후 배양액을 원심분리한 결과, 펠렛이 두 부분(노란색과 흰색)으로 분리되는 것을 관찰하였다. 기체 크로마토그래피 분석결과, 10-KSA와 10-HSA는 흰 펠렛에서만 검출되었다. 이는 strain DS5에 의한 생전환 생성물이 세포 밖으로 배출되며 간단한 원심분리 단계를 통해 세포로부터 쉽게 회수될 수 있음을 나타낸다.

Flavobacterium sp. strain DS5(NRRL B-14859) was used to convert oleic acid to 10-ketostearic acid(10-KSA) via 10-hydroxystearic acid(10-HSA). Increase in cell concentration by centrifuging, collecting cells grown in two flasks, and resuspending in one flask, improved 10-KSA production to 6.5 g/L from 3.5 g/L in a usual flask culture. Tween-80 addition to the culture did not greatly affect the production of 10-KSA and 10-HSA. When culture broth was centrifuged after fermentation, it was observed that pellets were separated into two parts(yellow and white). Gas chromatography analysis showed that 10-KSA and 10-HSA were detected only in a white pellet, suggesting that the bioconversion products of strain DS5 are extracellularly produced and can be easily recovered from cells by a simple centrifugation step.

Keywords:Oxygenated Fatty Acids;Flavobacterium sp. Strain DS5;10-Ketostearic Acid;10-Hydroxystearic Acid;Oleic Acid

[References]

Hou CT, Hosokawa M, in Hou CT(Ed.), Handbook of Industrial Biocatalysis, CRC Press, 7-1~7-25 (2005)
Kim BS, Song BS, Hou CT, John Wiley & Sons, 547 (2008)
Hou CT, Appl. Environ. Microbiol., 60, 3760 (1994)
Hou CT, J. Am. Oil Chem. Soc., 71, 975 (1994)
Hou CT, J. Ind. Microbiol., 14, 31 (1995)
Song BS, Han NS, Lee BH, Hou CT, Kim BS, Kor. Soc. Biotechnol. Bioeng. J., 24, 41 (2009)
Heo SH, Hou CT, Kim BS, New Biotechnol., doi:10.1016/j.nbt.2009.03.003 (2009)
Dalmau E, Montesinos JL, Lotti M, Casas C, Enzyme Microb. Technol., 26(9-10), 657 (2000)
Cheng MH, Walker TH, Hulbert GJ, Raman DR, Bioresour. Technol., 67(2), 101 (1999)
Kim BS, Kim HR, Hou CT, New Biotechnol., doi:10.1016/j.nbt.2009.09.002 (2009)

[Related Articles]

[1] Hou CT, Hosokawa M, in Hou CT(Ed.), Handbook of Industrial Biocatalysis, CRC Press, 7-1~7-25 (2005)

[2] Kim BS, Song BS, Hou CT, John Wiley & Sons, 547 (2008)

[3] Hou CT, Appl. Environ. Microbiol., 60, 3760 (1994)

[4] Hou CT, J. Am. Oil Chem. Soc., 71, 975 (1994)

[5] Hou CT, J. Ind. Microbiol., 14, 31 (1995)

[6] Song BS, Han NS, Lee BH, Hou CT, Kim BS, Kor. Soc. Biotechnol. Bioeng. J., 24, 41 (2009)

[7] Heo SH, Hou CT, Kim BS, New Biotechnol., doi:10.1016/j.nbt.2009.03.003 (2009)

[8] Dalmau E, Montesinos JL, Lotti M, Casas C, Enzyme Microb. Technol., 26(9-10), 657 (2000)

[9] Cheng MH, Walker TH, Hulbert GJ, Raman DR, Bioresour. Technol., 67(2), 101 (1999)

[10] Kim BS, Kim HR, Hou CT, New Biotechnol., doi:10.1016/j.nbt.2009.09.002 (2009)