The two-photon absorption properties of bisfluorene dendrimers are investigated in both the nanosecond and the femtosecond regime. By substituting different dendrons onto the same chromophore (see figure), we examine the effect of the dendrons on the spectrum and magnitude of the two-photon absorption. As these dendrimers have high photoluminescence quantum yields, we then proceed to make two-photon pumped dendrimer lasers.