The [PtCl2(LL)] complex, as a cisplatin derivative, which LL is diamine chelate ligand (N,N-dimethyltrimethylendiamine), was synthesized and characterized by elemental analysis (CHN) mass, H-1, and C-13 nuclear magnetic resonance techniques. Then the binding of this complex to calf thymus DNA was investigated by various physicochemical methods such as spectrophotometric, circular dichroism, spectrofluorometric, melting temperature, and viscosimetric techniques. Upon addition of the complex, important changes were observed in the characteristic UV-Vis bands (hypochromism) of calf thymus DNA, increase in melting temperature and some changes in specific viscosity. Also, the fluorescence spectral characteristics showed an increase in the fluorescence intensity of methylene blue-DNA solutions in the presence of increasing amounts of metal complex, indicating PtCl2(LL) is able to displace the methylene blue bound to DNA but not as complete as intercalative molecules. The experimental results showed that the platinum complex is bound to DNA non-intercalatively, and an outside binding is the preferred mode of interaction.