Several methods have been employed in recent years to investigate homogeneous surface topography based on image analysis, such as AFM (atomic force microscopy) and SEM (scanning electron microscopy). Fractal analysis of the images provides fractal dimension of the surface which is used as one of the most common surface indices. Surface topography has generally been considered to be mono-fractal. On the other hand, precipitation of organic materials on a rough surface and its irregular growth result in morphology alteration and converts a homogeneous surface to a heterogeneous one. In this case a mono-fractal description of the surface does not completely describe the nature of the altered surface. This work aims to investigate the topography alteration of a glass surface as a result of asphaltene precipitation and its growth at various pressures using a bi-fractal approach. The experimental results of the deposited surfaces were clearly indicating two regions of micro- and macro-asperities namely, surface types I and II, respectively. The fractal plots were indicative of bi-fractal behavior and for each surface type one fractal dimension was calculated. The topography information of the surfaces was obtained by two image analyses, AFM and SEM imaging techniques. Results of the bi-fractal analysis demonstrated that topography alteration in surface type II (macro-asperities) is more evident than that in surface type I ( micro- asperities). Compared to surface type II, a better correlation was observed between the fractal dimensions inferred from the AFM images (D-A) and those of the SEM images (D-S) in surface type I. (C) 2009 Elsevier B. V. All rights reserved.