The preparation of silver nanoparticles (AgNPs) is of great interest due to their various biological activities, Such as observed in their antimicrobial and Wound healing actions. Moreover, the formation of AgNPs using silver-binding peptide has certain advantages because they can be made in aqueous Solution at ambient temperature. The solution Structure of the silver-binding peptide AG4 was determined using nuclear magnetic resonance spectroscopy, and the site of the AG4 interaction with AgNPs was elucidated. (C) 2008 Elsevier Inc. All rights reserved.