We have developed a novel micro-mixer using a biological molecular ATP motor. The micro-mixer was Constructed from arrays of chromatophore-embedded delta-free F0F1-ATPases, where the delta-free F-1 part acted as a rotator to Mix solutions, and the F-0 part was driven by light. Confocal microscope Studies indicated that the micro-mixer did not touch directly oil the fibrin labeled with FITC. The nanomechanical force generated by the motor induced drug movement in the solution and accelerated the fibrinolysis process. All results strongly Suggest that the micro-mixers generated a nanomechanical force which accelerated the fibrinolysis process in the presence of lower concentrations of lumbrokinase.. Crown Copyright (C) 2008 Published by Elsevier Inc. All rights reserved.