Potassium tellurite (K2TeO3) is harmful to most organisms and specific mechanisms explaining its toxicity are not well known to date. We previously reported that the lpdA gene product of the tellurite-resistant environmental isolate Aeromonas caviae ST is involved in the reduction of tellurite to elemental tellurium. In this work, we show that expression of A. caviae ST aceE, aceF, and IpdA genes, encoding pyruvate dehydrogenase, dihydrolipoamide transacetylase, and dihydrolipoamide dehydrogenase, respectively, results in tellurite resistance and decreased levels of tellurite-induced Superoxide in Escherichia coli. In addition to oxidative damage resulting from tellurite exposure, a metabolic disorder Would be simultaneously established in which the pyruvate dehydrogenase complex would represent an intracellular tellurite target. These results allow LIS to widen Our Vision regarding the molecular mechanisms involved in bacterial tellurite resistance by correlating tellurite toxicity and key enzymes of aerobic metabolism. (C) 2009 Elsevier Inc. All rights reserved.