PEGA/BMA 공중합체의 코팅을 통해 CD34 단일클론항체가 고정화된 폴리우레탄 표면

정윤기; 황인규; 박기동; Yoon Ki Joung; In Kyu Hwang; Ki Dong Park

Polymer(Korea), Vol.33, No.6, 602-607, November, 2009

Export Citation

PEGA/BMA 공중합체의 코팅을 통해 CD34 단일클론항체가 고정화된 폴리우레탄 표면

CD34 Monoclonal Antibody-Immobilization on Polyurethane Surface by Poly(PEGA-co-BMA) Coating

정윤기, 황인규, 박기동^†

아주대학교 분자과학기술학과

Yoon Ki Joung, In Kyu Hwang, and Ki Dong Park^†

Department of Molecular Science and Technology, Ajou University, 5 Wonchon, Yeongtong, Suwon 443-749, Korea

E-mail:

초록

심혈관용 소재로서 혈관내피전구세포의 포획을 통해 in vivo 내피세포화가 가능한 표면을 가진 폴리우레탄 표면을 개발하였다. 혈관내피전구세포의 점착을 유도하는 CD34 단일클론항체(monoclonal antibody, mAb)를 표면에 도입하기 위해, poly(poly(ethylene glycol) acrylate-co-butyl methacrylate), poly(PEGA-co-BMA) 공중합체가 합성되었고, 이를 폴리우레탄 표면에 코팅하여 CD34 단일클론항체를 화학적으로 고정화하였다. 중합된 공중합체의 1HNMR 분석은 원하는 조성을 가진 poly(PEGA-co-BMA)의 합성이 가능함을 확인해 주었다. 이전 연구에서 개발된 PEG가 그래프트된 폴리우레탄과의 비교를 통해, 본 연구에서 제조된 poly(PEGA-co-BMA)가 코팅된 폴리우레탄 표면이 CD34 mAb의 고정화에 더 효과적인 것으로 나타났으며, 이는 CD34 mAb의 표면밀도와 활성도가 32배 이상 증가된 결과를 통해 증명되었다. 개질된 폴리우레탄 표면의 물리화학적 특성은 XPS와 물 접촉각, AFM에 의해 분석되었으며, 각각의 개질된 표면에 따른 표면의 특이적 성질을 잘 보여주었다. 본 연구에서 얻어진 결과들은 poly(PEGAco-BMA)의 코팅을 통해 제조된 표면이 CD34 mAb의 고정화에 효과적임을 설명하였으며, 실제로 심혈관용 소재의 개발에 적용 가능성이 크다는 것을 증명해 주었다.

A polyurethane (PU) surface enabling in vivo endothelialization via endothelial progenitor cell (EPC) capture was prepared for cardiovascular applications. To introduce CD34 monoclonal antibody (mAb) inducing EPC adhesion onto a surface, poly(poly(ethylene glycol) acrylate-co-butyl methacrylate) and poly (PEGA-co-BMA) were synthesized and then coated on a surface of PU, followed by immobilizing CD34 mAb. 1H-NMR analysis demonstrated that poly(PEGA-co-BMA) copolymers with a desired composition were synthesized. Poly(PEGA-co-BMA)-coated PU was much more effective for the immobilization of CD34 mAb, comparing with PEG-grafted PU prepared in our previous study, as demonstrated by that surface density and activity of CD34 mAb increased over 32 times. Physico-chemical properties of modified PU surfaces were characterized by X-ray photoelectron spectroscopy (XPS), water contact angle, and atomic force microscopy (AFM). The results demonstrated that the poly(PEGA-co-BMA) coating was effective for CD34 mAb immobilization and feasible for applying to cardiovascular biomaterials.

Keywords:surface immobilization;CD34 monoclonal antibody;polyurethane;endothelial progenitor cell;endothelialization

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Larsen CC, Kligman F, Kottke-Marchant K, Marchant RE, Biomaterials, 27, 4846 (2006)
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[1] Davidson MH, Am. J. Manag. Care, 13, S260 (2007)

[2] Rosamond W, Flegal K, Furie K, Go A, Greenlund K, Haase N, et al., Circulation, 117, 25 (2008)

[3] Bhargava B, Karthikeyan G, Abizaid AS, Mehran R, BMJ, 327, 274 (2003)

[4] Ward MR, Stewart DJ, Kutryk MJ, Catheter Cardiovasc. Interv., 70, 983 (2007)

[5] Sayers RD, Raptis S, Berce M, Miller JH, Br. J. Surg., 85, 934 (1998)

[6] Faries PL, Logerfo FW, Arora S, Hook S, Pulling MC, Akbari CM, et. al., J. Vasc. Surg., 32, 1080 (2000)

[7] Cines DB, Pollak ES, Buck CA, Loscalzo J, Zimmerman GA, McEver RP, et al., Blood, 91, 3527 (1998)

[8] Colman RW, Hemostasis and thrombosis: basic principles and clinical practice, 4th ed., Philadelphia, Lippincott Williams and Wilkins (2000)

[9] Jaffe EA, Ann. N. Y. Acad. Sci., 454, 279 (1985)

[10] Rodgers GM, Faseb. J., 2, 116 (1988)

[11] Hersel U, Dahmen C, Kessler H, Biomaterials, 24, 4385 (2003)

[12] Vargo TG, Gardella JAJ, Calvert JM, Chen MS, Science, 262, 1711 (1993)

[13] Neff JA, Tresco PA, Caldwell KD, Biomaterials, 20, 2377 (1999)

[14] Larsen CC, Kligman F, Kottke-Marchant K, Marchant RE, Biomaterials, 27, 4846 (2006)

[15] Choi WS, Bae JW, Lim HR, Kwon IK, Joung YK, Park JC, Park KD, Macromol. Res., 16, 42 (2009)

[16] Choi WS, Bae JW, Lim HR, Joung YK, Park JC, Park KD, Biomed. Mater., 3, 044104 (2008)

[17] Sigwart U, Puel J, Mirkovitch V, Joffre F, Kappenberger L, N. Engl. J. Med., 316, 701 (1987)

[18] Avci-Adali M, Paul A, Ziemer G, Wendel HP, Biomaterials, 29, 3936 (2008)

[19] Deutsch M, Meinhart J, Fischlein T, Preiss P, Zilla P, Surgery, 126, 847 (1999)

[20] Meinhart JG, Deutsch M, Fischlein T, Howanietz N, Froschl A, Zilla P, Ann. Thorac. Surg., 71, S327 (2001)

[21] Zhu C, Ying D, Mi J, Li L, Zeng W, Hou C, Sun J, Yuan W, Wen C, Zhang W, Biomaterials, 29, 2628 (2008)

[22] Wilhelm C, Bal L, Smirnov P, Galy-Fauroux I, Clement O, Gazeau F, Emmerich J, Biomaterials, 28, 3797 (2007)

[23] Ben-Shoshan J, George J, Pharmacol. Therapeut., 115, 25 (2007)

[24] Fuchs S, Motta A, Migliaresi C, Kirkpatrick CJ, Biomaterials, 27, 5399 (2006)

[25] Park KD, Kim SW, Biomedical Application of Polyethylene Glycol Chemistry, M. Harris, Editor, Plenum Publ., New York, USA (1992)

[26] Han DK, Park KD, Kim YH, J. Biomater. Sci.: Polym. Edn., 9, 163 (1998)

[27] Park KD, Suzuki K, Lee WK, Lee JE, Kim YH, Sakurai Y, Okano T, ASAIO J., 42, 876 (1996)

[28] Han DK, Park KD, Ryu GH, Kim UY, Min BG, Kim YH, J. Biomed. Mater. Res., 30, 23 (1996)

[29] Han DK, Park KD, Jeong SY, Kim YH, Lee NY, Cho HI, Min BG, Biomaterials, 16, 467 (1995)