Ru-containing MCM-48 has been synthesized by a facile way without employing hydrothermal synthesis for the first time, and characterized by XRD, N-2 adsorption/desorption, UV-vis and HRTEM. The catalytic activity of the prepared Ru-MCM-48 in the oxidation of cyclohexane using oxygen as oxidant without any solvent was evaluated, and the results of the reaction indicate that Ru-MCM-48 is an efficient catalyst.