In order to understand better which parameters control the mass transfer efficiencies in biofilters, hydrodynamic and mass transfer parameters have been investigated for seven different packings in a three phase fixed bed reactor operating in co-current gas-liquid up flow. For this purpose, the packing characteristics (bed porosity, particle size and particle shape) and hydrodynamics parameters have been determined. Thus, the pressure drops, global gas hold-up, static gas fraction, slip velocities and bubble size have been investigated. For each type of solid, volumetric oxygen mass transfer coefficients have been measured under different operating conditions of gas (air) and liquid (tap water). The experiments were performed in a cylindrical PVC column with co-current up-flow of gas and liquid at laboratory temperature and atmospheric pressure. Air was injected at the bottom of the column using a porous disc diffuser. The superficial gas velocity ranged from 2.3E-3 m/s to 2.5E-2 m/s, the superficial liquid velocity varied from 1.2E-3 m/s to 3.3E-3 m/s. The column of 0.15 m in diameter and 4 m height was filled with packing. The following values were determined: Pressure drop through the bed height was measured using differential manometers. The average values of the global gas hold-up and static gas fraction were determined in the experimental set up by a new technique which is based on a monitoring of liquid flow. The bubble sizes and slip velocities were determined by image acquisition and data treatment systems on a particular glass cell. The volumetric oxygen mass transfer coefficient was determined by gas and liquid mass balance techniques. These different techniques applied on the fixed bed bubble column enabled for each type of solid a complete characterisation of hydrodynamic and mass transfer to be described. These experimental tools were used to compare and evaluate the influence of solid characteristics on the reactor performances and to initiate a mass transfer model. (c) 2007 Elsevier B.V. All rights reserved.